DNA polymorphism identity determination using flow cytometry
Abstract
DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.
- Inventors:
-
- Santa Fe, NM
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 873983
- Patent Number(s):
- 6287766
- Assignee:
- Regents of University of California (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dna; polymorphism; identity; determination; flow; cytometry; primers; designed; immobilized; microspheres; allowed; anneal; strand; investigation; extended; polymerase; fluorescent; dideoxynucleotides; ligated; ligase; reporter; oligonucleotides; fluorescence; dideoxynucleotide; oligonucleotide; attached; primer; measured; identifying; nucleotide; dna strand; dna polymerase; flow cytometry; dna ligase; identity determination; polymorphism identity; dna polymorphism; /435/999/935/
Citation Formats
Nolan, John P, White, P Scott, and Cai, Hong. DNA polymorphism identity determination using flow cytometry. United States: N. p., 2001.
Web.
Nolan, John P, White, P Scott, & Cai, Hong. DNA polymorphism identity determination using flow cytometry. United States.
Nolan, John P, White, P Scott, and Cai, Hong. Mon .
"DNA polymorphism identity determination using flow cytometry". United States. https://www.osti.gov/servlets/purl/873983.
@article{osti_873983,
title = {DNA polymorphism identity determination using flow cytometry},
author = {Nolan, John P and White, P Scott and Cai, Hong},
abstractNote = {DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
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