Recombinant organisms capable of fermenting cellobiose
Abstract
This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate-dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose, including cellobiose, in the production of ethanol.
- Inventors:
-
- Gainesville, FL
- Issue Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- OSTI Identifier:
- 873150
- Patent Number(s):
- 6102690
- Assignee:
- Univ. of Florida Research Foundation, Inc. (Gainsville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-96ER20222
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- recombinant; organisms; capable; fermenting; cellobiose; relates; microorganism; expresses; pyruvate; decarboxylase; alcohol; dehydrogenase; klebsiella; phospho-; beta; -glucosidase; phosphoenolpyruvate-dependent; phosphotransferase; cellobiose-utilizing; enzyme; ii; heterologous; utilizing; hemicellulose; cellulose; including; production; ethanol; alcohol dehydrogenase; pyruvate decarboxylase; recombinant organisms; organisms capable; /431/435/999/
Citation Formats
Ingram, Lonnie O, Lai, Xiaokuang, Moniruzzaman, Mohammed, and York, Sean W. Recombinant organisms capable of fermenting cellobiose. United States: N. p., 2000.
Web.
Ingram, Lonnie O, Lai, Xiaokuang, Moniruzzaman, Mohammed, & York, Sean W. Recombinant organisms capable of fermenting cellobiose. United States.
Ingram, Lonnie O, Lai, Xiaokuang, Moniruzzaman, Mohammed, and York, Sean W. Sat .
"Recombinant organisms capable of fermenting cellobiose". United States. https://www.osti.gov/servlets/purl/873150.
@article{osti_873150,
title = {Recombinant organisms capable of fermenting cellobiose},
author = {Ingram, Lonnie O and Lai, Xiaokuang and Moniruzzaman, Mohammed and York, Sean W},
abstractNote = {This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate-dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose, including cellobiose, in the production of ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Works referenced in this record:
Discovery of a ptsHl operon, which includes a third gene (ptsT), in the thermophile Bacillus stearothermophilus
journal, June 1995
- Lai, X.; Ingram, L. O.
- Microbiology, Vol. 141, Issue 6
Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes.
journal, November 1987
- Saffen, D. W.; Presper, K. A.; Doering, T. L.
- Journal of Biological Chemistry, Vol. 262, Issue 33
Cloning and expression of a β-glucosidase gene from Xanthomonas albilineans in Escherichia coli and Zymomonas mobilis
journal, January 1989
- Su, Ping; Delaney, Stephen F.; Rogers, Peter L.
- Journal of Biotechnology, Vol. 9, Issue 2
Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides.
journal, January 1997
- Lai, X.; Davis, F. C.; Hespell, R. B.
- Applied and environmental microbiology, Vol. 63, Issue 2
The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli.
journal, March 1968
- Fox, C. F.; Wilson, G.
- Proceedings of the National Academy of Sciences, Vol. 59, Issue 3
Isolation and molecular characterization of high-performance cellobiose-fermenting spontaneous mutants of ethanologenic Escherichia coli KO11 containing the Klebsiella oxytoca casAB operon.
journal, January 1997
- Moniruzzaman, M.; Lai, X.; York, S. W.
- Applied and environmental microbiology, Vol. 63, Issue 12
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering
journal, February 1993
- Sprenger, Georg A.
- Journal of Biotechnology, Vol. 27, Issue 3
Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum.
journal, January 1992
- Wood, B. E.; Ingram, L. O.
- Applied and Environmental Microbiology, Vol. 58, Issue 7
Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli.
journal, January 1993
- Yomano, L. P.; Scopes, R. K.; Ingram, L. O.
- Journal of Bacteriology, Vol. 175, Issue 13
Expression and stability of a .BETA.-glucosidase gene of Ruminococcus albus in Zymomonas mobilis.
journal, January 1989
- Misawa, Norihiko; Nakamura, Katsumi
- Agricultural and Biological Chemistry, Vol. 53, Issue 3