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Title: Recombinant organisms capable of fermenting cellobiose

Abstract

This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate-dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose, including cellobiose, in the production of ethanol.

Inventors:
 [1];  [1];  [1];  [1]
  1. Gainesville, FL
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
OSTI Identifier:
873150
Patent Number(s):
6102690
Assignee:
Univ. of Florida Research Foundation, Inc. (Gainsville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
recombinant; organisms; capable; fermenting; cellobiose; relates; microorganism; expresses; pyruvate; decarboxylase; alcohol; dehydrogenase; klebsiella; phospho-; beta; -glucosidase; phosphoenolpyruvate-dependent; phosphotransferase; cellobiose-utilizing; enzyme; ii; heterologous; utilizing; hemicellulose; cellulose; including; production; ethanol; alcohol dehydrogenase; pyruvate decarboxylase; recombinant organisms; organisms capable; /431/435/999/

Citation Formats

Ingram, Lonnie O, Lai, Xiaokuang, Moniruzzaman, Mohammed, and York, Sean W. Recombinant organisms capable of fermenting cellobiose. United States: N. p., 2000. Web.
Ingram, Lonnie O, Lai, Xiaokuang, Moniruzzaman, Mohammed, & York, Sean W. Recombinant organisms capable of fermenting cellobiose. United States.
Ingram, Lonnie O, Lai, Xiaokuang, Moniruzzaman, Mohammed, and York, Sean W. Sat . "Recombinant organisms capable of fermenting cellobiose". United States. https://www.osti.gov/servlets/purl/873150.
@article{osti_873150,
title = {Recombinant organisms capable of fermenting cellobiose},
author = {Ingram, Lonnie O and Lai, Xiaokuang and Moniruzzaman, Mohammed and York, Sean W},
abstractNote = {This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate-dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose, including cellobiose, in the production of ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}

Works referenced in this record:

Cloning and expression of a β-glucosidase gene from Xanthomonas albilineans in Escherichia coli and Zymomonas mobilis
journal, January 1989


The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli.
journal, March 1968


Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli.
journal, January 1993


Expression and stability of a .BETA.-glucosidase gene of Ruminococcus albus in Zymomonas mobilis.
journal, January 1989