Title: Ethanol production by recombinant hosts

Abstract

Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.

Inventors:

Ingram, Lonnie O ^[1]; Beall, David S ^[1]; Burchhardt, Gerhard F. H. ^[1]; Guimaraes, Walter V ^[2]; Ohta, Kazuyoshi ^[3]; Wood, Brent E ^[1]; Shanmugam, Keelnatham T ^[1]

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+ Show Inventor Affiliations

1. Gainesville, FL
2. Vicosa, BR
3. Miyazaki, JP

Issue Date:

Sun Jan 01 00:00:00 EST 1995

Research Org.:

Univ. of Florida, Gainesville, FL (United States)

OSTI Identifier:

869925

Patent Number(s):

5424202

Assignee:

University of Florida (Gainesville, FL)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/52 - Genes encoding for enzymes or proenzymes
C12N9/0006 - {acting on CH-OH groups as donors
C12N9/248 - {Xylanases}
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/065 - {with microorganisms other than yeasts}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y101/01001 - Alcohol dehydrogenase

E - FIXED CONSTRUCTIONS E05 - LOCKS E05Y - INDEXING SCHEME RELATING TO HINGES OR OTHER SUSPENSION DEVICES FOR DOORS, WINDOWS OR WINGS AND DEVICES FOR MOVING WINGS INTO OPEN OR CLOSED POSITION, CHECKS FOR WINGS AND WING FITTINGS NOT OTHERWISE PROVIDED FOR, CONCERNED WITH THE FUNCTIONING OF THE WING

E05Y2900/132 - Doors

G - PHYSICS G06 - COMPUTING G06T - IMAGE DATA PROCESSING OR GENERATION, IN GENERAL

G06T1/00 - General purpose image data processing

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/847 - Erwinia
Y10S435/854 - Lactobacillus acidophilus

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DOE Contract Number:  

FG05-86ER13574

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

ethanol; production; recombinant; hosts; novel; plasmids; comprising; genes; code; alcohol; dehydrogenase; pyruvate; decarboxylase; described; transformed; coding; virtue; transformation; capable; producing; significant; amounts; fermentation; product; disclosed; methods; increasing; growth; reducing; accumulation; undesirable; metabolic; products; medium; host; oligosaccharides; encoding; polysaccharases; addition; oligomeric; feedstock; provided; method; enhancing; functional; proteins; overexpressing; adhb; process; designs; fermenting; oligosaccharide-containing; biomass; fermentation product; comprising genes; plasmids comprising; ethanol production; producing ethanol; significant amounts; alcohol dehydrogenase; recombinant host; recombinant hosts; pyruvate decarboxylase; undesirable metabolic; metabolic products; significant amount; growth medium; novel plasmids; functional proteins; genes coding; genes encoding; process design; /435/