skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recombinant host cells and media for ethanol production

Abstract

Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.

Inventors:
; ; ;
Issue Date:
Research Org.:
University of Florida Research Foundation, Inc., Gainesville, FL, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1124787
Patent Number(s):
8,652,817
Application Number:
11/922,631
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Wood, Brent E, Ingram, Lonnie O, Yomano, Lorraine P, and York, Sean W. Recombinant host cells and media for ethanol production. United States: N. p., 2014. Web.
Wood, Brent E, Ingram, Lonnie O, Yomano, Lorraine P, & York, Sean W. Recombinant host cells and media for ethanol production. United States.
Wood, Brent E, Ingram, Lonnie O, Yomano, Lorraine P, and York, Sean W. Tue . "Recombinant host cells and media for ethanol production". United States. https://www.osti.gov/servlets/purl/1124787.
@article{osti_1124787,
title = {Recombinant host cells and media for ethanol production},
author = {Wood, Brent E and Ingram, Lonnie O and Yomano, Lorraine P and York, Sean W},
abstractNote = {Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {2}
}

Patent:

Save / Share:

Works referenced in this record:

Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration
journal, July 2001

  • Zaldivar, J.; Nielsen, J.; Olsson, L.
  • Applied Microbiology and Biotechnology, Vol. 56, Issue 1-2
  • DOI: 10.1007/s002530100624

Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli
journal, September 1994

  • von Sivers, Margareta; Zacchi, Guido; Olsson, Lisbeth
  • Biotechnology Progress, Vol. 10, Issue 5
  • DOI: 10.1021/bp00029a017

Potential Synergies and Challenges in Refining Cellulosic Biomass to Fuels, Chemicals, and Power
journal, April 2003

  • Wyman, C.E.
  • Biotechnology Progress, Vol. 19, Issue 2, p. 254-262
  • DOI: 10.1021/bp025654l

Fermentation of L-arabinose, D-xylose and D-glucose by ethanologenic recombinant Klebsiella oxytoca strain P2
journal, April 1994

  • Bothast, RodneyJ.; Saha, BadalC.; Flosenzier, A. Vance
  • Biotechnology Letters, Vol. 16, Issue 4
  • DOI: 10.1007/BF00245060

Saccharification and fermentation of Sugar Cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway
journal, June 1994

  • Doran, Joy B.; Aldrich, H. C.; Ingram, L. O.
  • Biotechnology and Bioengineering, Vol. 44, Issue 2, p. 240-247
  • DOI: 10.1002/bit.260440213

Conversion of Mixed Waste Office Paper to Ethanol by Genetically Engineered Klebsiella oxytoca Strain P2
journal, November 1995

  • Brooks, Todd A.; Ingram, L. O.
  • Biotechnology Progress, Vol. 11, Issue 6
  • DOI: 10.1021/bp00036a003

Ultrasound Stimulates Ethanol Production during the Simultaneous Saccharification and Fermentation of Mixed Waste Office Paper
journal, June 1997

  • Wood, B. E.; Aldrich, H. C.; Ingram, L. O.
  • Biotechnology Progress, Vol. 13, Issue 3
  • DOI: 10.1021/bp970027v

Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures
journal, April 1995

  • Lindsay, S. E.; Bothast, R. J.; Ingram, L. O.
  • Applied Microbiology and Biotechnology, Vol. 43, Issue 1, p. 70-75
  • DOI: 10.1007/BF00170625

Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production
journal, February 1998

  • Yomano, L. P.; York, S. W.; Ingram, L. O.
  • Journal of Industrial Microbiology and Biotechnology, Vol. 20, Issue 2
  • DOI: 10.1038/sj.jim.2900496

Cloning, Characterization, and Functional Expression of the Klebsiella oxytoca Xylodextrin Utilization Operon (xynTB) in Escherichia coli
journal, October 2003


Chemically defined media for commercial fermentations
journal, April 1999

  • Zhang, J.; Greasham, R.
  • Applied Microbiology and Biotechnology, Vol. 51, Issue 4
  • DOI: 10.1007/s002530051411