Recombinant host cells and media for ethanol production
Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.
- Issue Date:
- OSTI Identifier:
- University of Florida Research Foundation, Inc. (Gainesville, FL) CHO
- Patent Number(s):
- Application Number:
- Contract Number:
- Research Org:
- University of Florida Research Foundation, Inc., Gainesville, FL, USA
- Sponsoring Org:
- Country of Publication:
- United States
- 60 APPLIED LIFE SCIENCES
Other works cited in this record:Potential Synergies and Challenges in Refining Cellulosic Biomass to Fuels, Chemicals, and Power
journal, April 2003
- Wyman, C.E.
- Biotechnology Progress, Vol. 19, Issue 2, p. 254-262
Saccharification and fermentation of Sugar Cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway
journal, June 1994
- Doran, Joy B.; Aldrich, H. C.; Ingram, L. O.
- Biotechnology and Bioengineering, Vol. 44, Issue 2, p. 240-247
Cloning, Characterization, and Functional Expression of the Klebsiella oxytoca Xylodextrin Utilization Operon (xynTB) in Escherichia coli
journal, October 2003
- Qian, Y.; Yomano, L. P.; Preston, J. F.
- Applied and Environmental Microbiology, Vol. 69, Issue 10, p. 5957-5967
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