Absorption-emission optrode and methods of use thereof
Abstract
A method and apparatus for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance.
- Inventors:
-
- Livermore, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 867412
- Patent Number(s):
- 4929561
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- absorption-emission; optrode; methods; method; apparatus; monitoring; physical; chemical; properties; sample; fluid; measuring; optical; signal; generated; fluorescent; substance; modulated; absorber; emission; band; overlaps; absorption; degree; overlap; dependent; immobilized; substrate; effective; molecules; sufficiently; close; resonant; energy; transfer; occur; providing; highly; efficient; modulation; emissions; absorption band; energy transfer; sample fluid; signal generated; highly efficient; optical signal; fluorescent emissions; chemical properties; fluorescent emission; /436/250/422/
Citation Formats
Hirschfeld, Tomas B. Absorption-emission optrode and methods of use thereof. United States: N. p., 1990.
Web.
Hirschfeld, Tomas B. Absorption-emission optrode and methods of use thereof. United States.
Hirschfeld, Tomas B. Mon .
"Absorption-emission optrode and methods of use thereof". United States. https://www.osti.gov/servlets/purl/867412.
@article{osti_867412,
title = {Absorption-emission optrode and methods of use thereof},
author = {Hirschfeld, Tomas B},
abstractNote = {A method and apparatus for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1990},
month = {Mon Jan 01 00:00:00 EST 1990}
}
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