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Title: Hyperspectral stimulated emission depletion microscopy and methods of use thereof

A hyperspectral stimulated emission depletion ("STED") microscope system for high-resolution imaging of samples labeled with multiple fluorophores (e.g., two to ten fluorophores). The hyperspectral STED microscope includes a light source, optical systems configured for generating an excitation light beam and a depletion light beam, optical systems configured for focusing the excitation and depletion light beams on a sample, and systems for collecting and processing data generated by interaction of the excitation and depletion light beams with the sample. Hyperspectral STED data may be analyzed using multivariate curve resolution analysis techniques to deconvolute emission from the multiple fluorophores. The hyperspectral STED microscope described herein can be used for multi-color, subdiffraction imaging of samples (e.g., materials and biological materials) and for analyzing a tissue by Forster Resonance Energy Transfer ("FRET").
Inventors:
;
Issue Date:
OSTI Identifier:
1128705
Assignee:
Sandia Corporation (Albuquerque, NM) SNL
Patent Number(s):
8,686,363
Application Number:
13/609,057
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2012 Sep 10
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Other works cited in this record:

Method and apparatus for creating a topography at a surface
patent, November 2008

Förster resonance energy transfer – A spectroscopic nanoruler: Principle and applications
journal, March 2011

Hyperspectral confocal microscope
journal, January 2006
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In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells
journal, March 2008
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Preprocessing strategies to improve MCR analyses of hyperspectral images
journal, August 2012
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