Lux expression in eukaryotic cells

Gupta, Rakesh K; Patterson, Stacy S; Sayler, Gary S; Ripp, Steven A

Advanced Search OptionsAdvanced Search queries use a traditional Term Search. For more info, see our FAQ.

All Fields:

Patent Title:

Abstract:

Assignee:

Inventor(s):

Patent Number:

Patent Classification (CPC):

All Classifications
A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
A41 - wearing apparel
A42 - headwear
A43 - footwear
A44 - haberdashery
A45 - hand or travelling articles
A46 - brushware
A47 - furniture
A61 - medical or veterinary science
A62 - life-saving
A63 - sports
A99 - subject matter not otherwise provided for in this section
B - performing operations
B01 - physical or chemical processes or apparatus in general
B02 - crushing, pulverising, or disintegrating
B03 - separation of solid materials using liquids or using pneumatic tables or jigs
B04 - centrifugal apparatus or machines for carrying-out physical or chemical processes
B05 - spraying or atomising in general
B06 - generating or transmitting mechanical vibrations in general
B07 - separating solids from solids
B08 - cleaning
B09 - disposal of solid waste
B21 - mechanical metal-working without essentially removing material
B22 - casting
B23 - machine tools
B24 - grinding
B25 - hand tools
B26 - hand cutting tools
B27 - working or preserving wood or similar material
B28 - working cement, clay, or stone
B29 - working of plastics
B30 - presses
B31 - making articles of paper, cardboard or material worked in a manner analogous to paper
B32 - layered products
B33 - additive manufacturing technology
B41 - printing
B42 - bookbinding
B43 - writing or drawing implements
B44 - decorative arts
B60 - vehicles in general
B61 - railways
B62 - land vehicles for travelling otherwise than on rails
B63 - ships or other waterborne vessels
B64 - aircraft
B65 - conveying
B66 - hoisting
B67 - opening, closing {or cleaning} bottles, jars or similar containers
B68 - saddlery
B81 - microstructural technology
B82 - nanotechnology
B99 - subject matter not otherwise provided for in this section
C - chemistry
C01 - inorganic chemistry
C02 - treatment of water, waste water, sewage, or sludge
C03 - glass
C04 - cements
C05 - fertilisers
C06 - explosives
C07 - organic chemistry
C08 - organic macromolecular compounds
C09 - dyes
C10 - petroleum, gas or coke industries
C11 - animal or vegetable oils, fats, fatty substances or waxes
C12 - biochemistry
C13 - sugar industry
C14 - skins
C21 - metallurgy of iron
C22 - metallurgy
C23 - coating metallic material
C25 - electrolytic or electrophoretic processes
C30 - crystal growth
C40 - combinatorial technology
C99 - subject matter not otherwise provided for in this section
D - textiles
D01 - natural or man-made threads or fibres
D02 - yarns
D03 - weaving
D04 - braiding
D05 - sewing
D06 - treatment of textiles or the like
D07 - ropes
D10 - indexing scheme associated with sublasses of section d, relating to textiles
D21 - paper-making
D99 - subject matter not otherwise provided for in this section
E - fixed constructions
E01 - construction of roads, railways, or bridges
E02 - hydraulic engineering
E03 - water supply
E04 - building
E05 - locks
E06 - doors, windows, shutters, or roller blinds in general
E21 - earth drilling
E99 - subject matter not otherwise provided for in this section
F - mechanical engineering
F01 - machines or engines in general
F02 - combustion engines
F03 - machines or engines for liquids
F04 - positive - displacement machines for liquids
F05 - indexing schemes relating to engines or pumps in various subclasses of classes f01-f04
F15 - fluid-pressure actuators
F16 - engineering elements and units
F17 - storing or distributing gases or liquids
F21 - lighting
F22 - steam generation
F23 - combustion apparatus
F24 - heating
F25 - refrigeration or cooling
F26 - drying
F27 - furnaces
F28 - heat exchange in general
F41 - weapons
F42 - ammunition
F99 - subject matter not otherwise provided for in this section
G - physics
G01 - measuring
G02 - optics
G03 - photography
G04 - horology
G05 - controlling
G06 - computing
G07 - checking-devices
G08 - signalling
G09 - education
G10 - musical instruments
G11 - information storage
G12 - instrument details
G16 - information and communication technology [ict] specially adapted for specific application fields
G21 - nuclear physics
G99 - subject matter not otherwise provided for in this section
H - electricity
H01 - basic electric elements
H02 - generation
H03 - basic electronic circuitry
H04 - electric communication technique
H05 - electric techniques not otherwise provided for
H99 - subject matter not otherwise provided for in this section
Y - new / cross sectional technologies
Y02 - technologies or applications for mitigation or adaptation against climate change
Y04 - information or communication technologies having an impact on other technology areas
Y10 - technical subjects covered by former uspc

More Options ...

Title: Lux expression in eukaryotic cells

Abstract

The luxA, B, C, D, and E genes from Photorhabdus luminescens have been introduced into Saccharomyces cerevisiae bioluminescent yeast cells.

Inventors:

Gupta, Rakesh K ^[1]; Patterson, Stacy S ^[2]; Sayler, Gary S ^[3]; Ripp, Steven A ^[2]

New Delhi, IN
Knoxville, TN
Blaine, TN

Issue Date:: Tue Nov 27 00:00:00 EST 2007

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 920891

Patent Number(s):: 7300792

Application Number:: 10/408,003

Assignee:: University of Tennessee Research Foundation (Knoxville, TN)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/52 - Genes encoding for enzymes or proenzymes
C12N9/0071 - {acting on paired donors with incorporation of molecular oxygen

Show less

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Citation Formats


                    Gupta, Rakesh K, Patterson, Stacy S, Sayler, Gary S, and Ripp, Steven A. Lux expression in eukaryotic cells.  United States: N. p., 2007. 
        Web.

Copy to clipboard


                    Gupta, Rakesh K, Patterson, Stacy S, Sayler, Gary S, & Ripp, Steven A. Lux expression in eukaryotic cells.  United States.

Copy to clipboard


                    Gupta, Rakesh K, Patterson, Stacy S, Sayler, Gary S, and Ripp, Steven A. Tue .  
        "Lux expression in eukaryotic cells".  United States.  https://www.osti.gov/servlets/purl/920891.

Copy to clipboard


                    
@article{osti_920891,

  title        = {Lux expression in eukaryotic cells},

  author       = {Gupta, Rakesh K and Patterson, Stacy S and Sayler, Gary S and Ripp, Steven A},

  abstractNote = {The luxA, B, C, D, and E genes from Photorhabdus luminescens have been introduced into Saccharomyces cerevisiae bioluminescent yeast cells.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Nov 27 00:00:00 EST 2007},

  month        = {Tue Nov 27 00:00:00 EST 2007}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Reporter gene bioassays in environmental analysis
journal, March 2000

Köhler, S.; Belkin, S.; Schmid, R. D.
Fresenius' Journal of Analytical Chemistry, Vol. 366, Issue 6-7
https://doi.org/10.1007/s002160051571

Fatty acid synthesis in mitochondria from Saccharomyces cerevisiae
journal, April 1987

Bessoule, Jean-Jacques; Lessire, René; Rigoulet, Michel
FEBS Letters, Vol. 214, Issue 1
https://doi.org/10.1016/0014-5793(87)80033-9

Internal Initiation of Translation Directed by the 5′-Untranslated Region of the mRNA for eIF4G, a Factor Involved in the Picornavirus-induced Switch from Cap-dependent to Internal Initiation
journal, January 1996

Gan, Weiniu; Rhoads, Robert E.
Journal of Biological Chemistry, Vol. 271, Issue 2
https://doi.org/10.1074/jbc.271.2.623

Active bacterial luciferase from a fused gene: expression of a Vibrio harveyi luxAB translational fusion in bacteria, yeast and plant cells
journal, September 1989

Kirchner, G.; Roberts, J. L.; Gustafson, G. D.
Gene, Vol. 81, Issue 2
https://doi.org/10.1016/0378-1119(89)90195-9

Characterization of N-myristoyltransferase from Plasmodium falciparum
journal, May 2000

Gunaratne, Ruwani S.; Sajid, Mohammed; Ling, Irene T.
Biochemical Journal, Vol. 348, Issue 2
https://doi.org/10.1042/bj3480459

Internal ribosome entry sites in eukaryotic mRNA molecules
journal, July 2001

Hellen, C. U. T.
Genes & Development, Vol. 15, Issue 13
https://doi.org/10.1101/gad.891101

Endocrine disrupters — testing strategies to assess human hazard
journal, August 2001

Baker, V. A.
Toxicology in Vitro, Vol. 15, Issue 4-5
https://doi.org/10.1016/S0887-2333(01)00045-5

Engineering of monomeric bacterial luciferases by fusion of luxA and luxB genes in Vibrio harveyi
journal, September 1989

Olsson, Olof; Escher, Alan; Sandberg, Göran
Gene, Vol. 81, Issue 2
https://doi.org/10.1016/0378-1119(89)90194-7

Genetically Engineered Whole-Cell Sensing Systems: Coupling Biological Recognition with Reporter Genes
journal, July 2000

Daunert, Sylvia; Barrett, Gary; Feliciano, Jessika S.
Chemical Reviews, Vol. 100, Issue 7
https://doi.org/10.1021/cr990115p

Fusion ofluxA andluxB and its expression inE. coli, S. cerevisiae andD. melanogaster
journal, April 1990

Almashanu, S.; Musafia, B.; Hadar, R.
Journal of Bioluminescence and Chemiluminescence, Vol. 5, Issue 2
https://doi.org/10.1002/bio.1170050204

Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity
journal, December 1996

Henstrand, John M.; Schaller, Andreas; Braun, Martin
Molecular Microbiology, Vol. 22, Issue 5
https://doi.org/10.1046/j.1365-2958.1996.01534.x

Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae
journal, August 1998

Miller, C.
Nucleic Acids Research, Vol. 26, Issue 15
https://doi.org/10.1093/nar/26.15.3577

Crystal Structure and Functional Characterization of Yeast YLR011wp, an Enzyme with NAD(P)H-FMN and Ferric Iron Reductase Activities
journal, June 2004

Liger, Dominique; Graille, Marc; Zhou, Cong-Zhao
Journal of Biological Chemistry, Vol. 279, Issue 33
https://doi.org/10.1074/jbc.M405404200

Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen
journal, March 1996

Routledge, Edwin J.; Sumpter, John P.
Environmental Toxicology and Chemistry, Vol. 15, Issue 3
https://doi.org/10.1002/etc.5620150303

Similar Records in DOE Patents and OSTI.GOV collections:

Compositions and methods for the expression of selenoproteins in eukaryotic cells

Patent Gladyshev, Vadim [Lincoln, NE]; Novoselov, Sergey [Puschino, RU]

Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.
Full Text Available
Expression of eukaryotic polypeptides in chloroplasts

Patent Mayfield, Stephen P

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtainedmore » without RB47 binding site activation. « less
Full Text Available
Expression of eukaryotic polypeptides in chloroplasts

Patent Mayfield, Stephen P.

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtainedmore » without RB47 binding site activation. « less
Full Text Available
Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

Patent Gambhir, Sanjiv; Pritha, Ray

Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.
Full Text Available
Methods for transforming and expression screening of filamentous fungal cells with a DNA library

Patent Teter, Sarah; Lamsa, Michael; Cherry, Joel; ...

The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of themore » « less
Full Text Available

Similar Records

Title: Lux expression in eukaryotic cells

Abstract

Citation Formats

Reporter gene bioassays in environmental analysis journal, March 2000

Fatty acid synthesis in mitochondria from Saccharomyces cerevisiae journal, April 1987

Internal Initiation of Translation Directed by the 5′-Untranslated Region of the mRNA for eIF4G, a Factor Involved in the Picornavirus-induced Switch from Cap-dependent to Internal Initiation journal, January 1996

Active bacterial luciferase from a fused gene: expression of a Vibrio harveyi luxAB translational fusion in bacteria, yeast and plant cells journal, September 1989

Characterization of N-myristoyltransferase from Plasmodium falciparum journal, May 2000

Internal ribosome entry sites in eukaryotic mRNA molecules journal, July 2001

Endocrine disrupters — testing strategies to assess human hazard journal, August 2001

Engineering of monomeric bacterial luciferases by fusion of luxA and luxB genes in Vibrio harveyi journal, September 1989

Genetically Engineered Whole-Cell Sensing Systems: Coupling Biological Recognition with Reporter Genes journal, July 2000

Fusion ofluxA andluxB and its expression inE. coli, S. cerevisiae andD. melanogaster journal, April 1990

Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity journal, December 1996

Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae journal, August 1998

Crystal Structure and Functional Characterization of Yeast YLR011wp, an Enzyme with NAD(P)H-FMN and Ferric Iron Reductase Activities journal, June 2004

Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen journal, March 1996

Reporter gene bioassays in environmental analysis
journal, March 2000

Fatty acid synthesis in mitochondria from Saccharomyces cerevisiae
journal, April 1987

Internal Initiation of Translation Directed by the 5′-Untranslated Region of the mRNA for eIF4G, a Factor Involved in the Picornavirus-induced Switch from Cap-dependent to Internal Initiation
journal, January 1996

Active bacterial luciferase from a fused gene: expression of a Vibrio harveyi luxAB translational fusion in bacteria, yeast and plant cells
journal, September 1989

Characterization of N-myristoyltransferase from Plasmodium falciparum
journal, May 2000

Internal ribosome entry sites in eukaryotic mRNA molecules
journal, July 2001

Endocrine disrupters — testing strategies to assess human hazard
journal, August 2001

Engineering of monomeric bacterial luciferases by fusion of luxA and luxB genes in Vibrio harveyi
journal, September 1989

Genetically Engineered Whole-Cell Sensing Systems: Coupling Biological Recognition with Reporter Genes
journal, July 2000

Fusion ofluxA andluxB and its expression inE. coli, S. cerevisiae andD. melanogaster
journal, April 1990

Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity
journal, December 1996

Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae
journal, August 1998

Crystal Structure and Functional Characterization of Yeast YLR011wp, an Enzyme with NAD(P)H-FMN and Ferric Iron Reductase Activities
journal, June 2004

Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen
journal, March 1996