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Title: Compositions and methods for the expression of selenoproteins in eukaryotic cells

Abstract

Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.

Inventors:
 [1];  [2]
  1. Lincoln, NE
  2. Puschino, RU
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1067327
Patent Number(s):
8,273,549
Application Number:
US Patent Application 12/428,007
Assignee:
Board of Regents of the University of Nebraska (Lincoln, NE)
DOE Contract Number:  
FG07-02ID14380
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gladyshev, Vadim, and Novoselov, Sergey. Compositions and methods for the expression of selenoproteins in eukaryotic cells. United States: N. p., 2012. Web.
Gladyshev, Vadim, & Novoselov, Sergey. Compositions and methods for the expression of selenoproteins in eukaryotic cells. United States.
Gladyshev, Vadim, and Novoselov, Sergey. Tue . "Compositions and methods for the expression of selenoproteins in eukaryotic cells". United States. https://www.osti.gov/servlets/purl/1067327.
@article{osti_1067327,
title = {Compositions and methods for the expression of selenoproteins in eukaryotic cells},
author = {Gladyshev, Vadim and Novoselov, Sergey},
abstractNote = {Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {9}
}

Patent:

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