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Title: Expression of eukaryotic polypeptides in chloroplasts

Abstract

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Inventors:
Issue Date:
Research Org.:
Scripps Research Institute
Sponsoring Org.:
USDOE
OSTI Identifier:
1176724
Patent Number(s):
RE44266
Application Number:
11/197,730
Assignee:
Scripps Research Institute
DOE Contract Number:  
FG03-93ER20116
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts. United States: N. p., 2013. Web.
Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts. United States.
Mayfield, Stephen P. Tue . "Expression of eukaryotic polypeptides in chloroplasts". United States. https://www.osti.gov/servlets/purl/1176724.
@article{osti_1176724,
title = {Expression of eukaryotic polypeptides in chloroplasts},
author = {Mayfield, Stephen P.},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {6}
}

Patent:

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