Expression of eukaryotic polypeptides in chloroplasts
Abstract
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
- Inventors:
- Issue Date:
- Research Org.:
- Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176724
- Patent Number(s):
- RE44266
- Application Number:
- 11/197,730; 6,156,517
- Assignee:
- The Scripps Research Institutes (La Jolla, CA)
- DOE Contract Number:
- FG03-93ER20116
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2005 Aug 03
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts. United States: N. p., 2013.
Web.
Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts. United States.
Mayfield, Stephen P. Tue .
"Expression of eukaryotic polypeptides in chloroplasts". United States. https://www.osti.gov/servlets/purl/1176724.
@article{osti_1176724,
title = {Expression of eukaryotic polypeptides in chloroplasts},
author = {Mayfield, Stephen P.},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {6}
}
Works referenced in this record:
Single polarization transmission in pedestal-supported silicon waveguides
journal, January 2011
- Cheng, Jierong; Zhang, Wei; Zhou, Qiang
- Optics Letters, Vol. 36, Issue 10