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Title: Prolonged cold storage of red blood cells by oxygen removal and additive usage

Abstract

Prolonged cold storage of red blood cells by oxygen removal and additive usage. A cost-effective, 4.degree. C. storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. The improved in vivo survival and the preservation of adenosine triphosphate levels, along with reduction in hemolysis and membrane vesicle production of red blood cells stored at 4.degree. C. for prolonged periods of time, is achieved by reducing the oxygen level therein at the time of storage; in particular, by flushing the cells with an inert gas, and storing them in an aqueous solution which includes adenine, dextrose, mannitol, citrate ion, and dihydrogen phosphate ion, but no sodium chloride, in an oxygen-permeable container which is located in an oxygen-free environment containing oxygen-scavenging materials.

Inventors:
 [1];  [2]
  1. (Boston, MA)
  2. (Newton, MA)
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
871751
Patent Number(s):
5789151
Assignee:
Regents of University of California (Los Alamos, NM) LANL
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
prolonged; cold; storage; blood; cells; oxygen; removal; additive; usage; cost-effective; degree; procedure; preserves; cell; quality; prolongs; post-transfusion; vivo; survival; described; improved; preservation; adenosine; triphosphate; levels; reduction; hemolysis; membrane; vesicle; production; stored; periods; time; achieved; reducing; level; therein; particular; flushing; inert; gas; storing; aqueous; solution; adenine; dextrose; mannitol; citrate; dihydrogen; phosphate; sodium; chloride; oxygen-permeable; container; located; oxygen-free; environment; containing; oxygen-scavenging; materials; cold storage; environment containing; sodium chloride; blood cells; aqueous solution; inert gas; containing oxygen; blood cell; prolonged period; oxygen removal; prolonged periods; storage procedure; cell quality; hydrogen ph; prolonged cold; additive usage; /435/604/

Citation Formats

Bitensky, Mark W., and Yoshida, Tatsuro. Prolonged cold storage of red blood cells by oxygen removal and additive usage. United States: N. p., 1998. Web.
Bitensky, Mark W., & Yoshida, Tatsuro. Prolonged cold storage of red blood cells by oxygen removal and additive usage. United States.
Bitensky, Mark W., and Yoshida, Tatsuro. Thu . "Prolonged cold storage of red blood cells by oxygen removal and additive usage". United States. https://www.osti.gov/servlets/purl/871751.
@article{osti_871751,
title = {Prolonged cold storage of red blood cells by oxygen removal and additive usage},
author = {Bitensky, Mark W. and Yoshida, Tatsuro},
abstractNote = {Prolonged cold storage of red blood cells by oxygen removal and additive usage. A cost-effective, 4.degree. C. storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. The improved in vivo survival and the preservation of adenosine triphosphate levels, along with reduction in hemolysis and membrane vesicle production of red blood cells stored at 4.degree. C. for prolonged periods of time, is achieved by reducing the oxygen level therein at the time of storage; in particular, by flushing the cells with an inert gas, and storing them in an aqueous solution which includes adenine, dextrose, mannitol, citrate ion, and dihydrogen phosphate ion, but no sodium chloride, in an oxygen-permeable container which is located in an oxygen-free environment containing oxygen-scavenging materials.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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