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Title: Detection and differentiation of coxiella burnetii in biological fluids

Abstract

Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

Inventors:
 [1];  [2];  [3];  [4]
  1. (Richland, WA)
  2. (Moscow, ID)
  3. (Albuquerque, NM)
  4. (Pullman, WA)
Issue Date:
Research Org.:
BATTELLE MEMORIAL INSTITUTE
OSTI Identifier:
867154
Patent Number(s):
4876186
Assignee:
Battelle Development Corporation (Columbus, OH) PNNL
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; differentiation; coxiella; burnetii; biological; fluids; methods; detecting; presence; samples; method; differentiating; strains; capable; causing; acute; disease; chronic; disclosed; comprise; treating; cells; contained; sample; expose; cellular; dna; hybridizing; specifically; rickettsial; burnetii-specific; labeled; probe; radioisotope; biotin; labels; preferred; allowing; autoradiography; colorimetric; assays; respectively; biological samples; labeled dna; cellular dna; biological fluids; treating cells; strains capable; biological fluid; comprise treating; acute disease; causing acute; causing chronic; colorimetric assays; coxiella burnetii; biological sample; cells contained; chronic disease; colorimetric assay; dna probe; expose cellular; differentiating strains; cells contain; /435/436/999/

Citation Formats

Frazier, Marvin E., Mallavia, Louis P., Baca, Oswald G., and Samuel, James E. Detection and differentiation of coxiella burnetii in biological fluids. United States: N. p., 1989. Web.
Frazier, Marvin E., Mallavia, Louis P., Baca, Oswald G., & Samuel, James E. Detection and differentiation of coxiella burnetii in biological fluids. United States.
Frazier, Marvin E., Mallavia, Louis P., Baca, Oswald G., and Samuel, James E. Sun . "Detection and differentiation of coxiella burnetii in biological fluids". United States. https://www.osti.gov/servlets/purl/867154.
@article{osti_867154,
title = {Detection and differentiation of coxiella burnetii in biological fluids},
author = {Frazier, Marvin E. and Mallavia, Louis P. and Baca, Oswald G. and Samuel, James E.},
abstractNote = {Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1989},
month = {1}
}

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