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Title: Detection and differentiation of coxiella burnetii in biological fluids

Abstract

Methods for detecting the presence of Coxiella burenetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

Inventors:
 [1];  [2];  [3];  [4]
  1. Richland, WA
  2. Moscow, ID
  3. Pullman, WA
  4. Albuquerque, NM
Issue Date:
Research Org.:
Battelle Memorial Institute, Columbus, OH (United States)
OSTI Identifier:
867250
Patent Number(s):
4895795
Assignee:
Battelle Development Corporation (Columbus, OH)
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; differentiation; coxiella; burnetii; biological; fluids; methods; detecting; presence; burenetii; samples; method; differentiating; strains; capable; causing; acute; disease; chronic; disclosed; comprise; treating; cells; contained; sample; expose; cellular; dna; hybridizing; specifically; rickettsial; burnetii-specific; labeled; probe; radioisotope; biotin; labels; preferred; allowing; autoradiography; colorimetric; assays; respectively; biological samples; labeled dna; cellular dna; biological fluids; treating cells; strains capable; biological fluid; comprise treating; acute disease; causing acute; causing chronic; colorimetric assays; coxiella burnetii; biological sample; cells contained; chronic disease; colorimetric assay; dna probe; expose cellular; differentiating strains; cells contain; /435/436/999/

Citation Formats

Frazier, Marvin E, Mallavia, Louis P, Samuel, James E, and Baca, Oswald G. Detection and differentiation of coxiella burnetii in biological fluids. United States: N. p., 1990. Web.
Frazier, Marvin E, Mallavia, Louis P, Samuel, James E, & Baca, Oswald G. Detection and differentiation of coxiella burnetii in biological fluids. United States.
Frazier, Marvin E, Mallavia, Louis P, Samuel, James E, and Baca, Oswald G. Mon . "Detection and differentiation of coxiella burnetii in biological fluids". United States. https://www.osti.gov/servlets/purl/867250.
@article{osti_867250,
title = {Detection and differentiation of coxiella burnetii in biological fluids},
author = {Frazier, Marvin E and Mallavia, Louis P and Samuel, James E and Baca, Oswald G},
abstractNote = {Methods for detecting the presence of Coxiella burenetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

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