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Title: Detection and differentiation of coxiella burnetii in biological fluids

Abstract

Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA with a DNA probe containing DNA sequences that specifically hybridize with C. burnetii DNA of strains associated with the capacity to cause acute or chronic disease.

Inventors:
 [1];  [2];  [3];  [4]
  1. Richland, WA
  2. Moscow, ID
  3. Derwood, MD
  4. Albuquerque, NM
Issue Date:
Research Org.:
Battelle Memorial Institute, Columbus, OH (United States)
OSTI Identifier:
868995
Patent Number(s):
5258283
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; differentiation; coxiella; burnetii; biological; fluids; methods; detecting; presence; samples; method; differentiating; strains; capable; causing; acute; disease; chronic; disclosed; comprise; treating; cells; contained; sample; expose; cellular; dna; hybridizing; probe; containing; sequences; specifically; hybridize; associated; capacity; biological samples; dna sequences; dna sequence; cellular dna; biological fluids; treating cells; strains capable; biological fluid; comprise treating; acute disease; causing acute; causing chronic; coxiella burnetii; biological sample; cells contained; chronic disease; dna probe; expose cellular; differentiating strains; cells contain; /435/436/999/

Citation Formats

Frazier, Marvin E, Mallavia, Louis P, Samuel, James E, and Baca, Oswald G. Detection and differentiation of coxiella burnetii in biological fluids. United States: N. p., 1993. Web.
Frazier, Marvin E, Mallavia, Louis P, Samuel, James E, & Baca, Oswald G. Detection and differentiation of coxiella burnetii in biological fluids. United States.
Frazier, Marvin E, Mallavia, Louis P, Samuel, James E, and Baca, Oswald G. Fri . "Detection and differentiation of coxiella burnetii in biological fluids". United States. https://www.osti.gov/servlets/purl/868995.
@article{osti_868995,
title = {Detection and differentiation of coxiella burnetii in biological fluids},
author = {Frazier, Marvin E and Mallavia, Louis P and Samuel, James E and Baca, Oswald G},
abstractNote = {Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA with a DNA probe containing DNA sequences that specifically hybridize with C. burnetii DNA of strains associated with the capacity to cause acute or chronic disease.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1993},
month = {1}
}

Works referenced in this record:

Chronic Q Fever: 1. Clinical Aspects of a Patient with Endocarditis
journal, November 1959


Q fever and Coxiella burnetii: a model for host-parasite interactions
journal, June 1983


Phase Variation of the nine mile and Other Strains of Rickettsia Burneti
journal, May 1956


Q fever endocarditis
journal, March 1982


Isolation and characterization of a plasmid from phase I Coxiella burnetii
journal, August 1983


Correlation of plasmid type and disease caused by Coxiella burnetii
journal, September 1985


Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.
journal, July 1983