Apparatus and method of determining molecular weight of large molecules
Abstract
A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e{sup {minus}} are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation. 14 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 672527
- Patent Number(s):
- 5770857
- Application Number:
- PAN: 8-749,837
- Assignee:
- Univ. of California, Oakland, CA (United States)
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 23 Jun 1998
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 40 CHEMISTRY; MASS SPECTROMETERS; MOLECULAR WEIGHT; DESIGN; OPERATION; MASS
Citation Formats
Fuerstenau, S, Benner, W H, Madden, N M, and Searles, W. Apparatus and method of determining molecular weight of large molecules. United States: N. p., 1998.
Web.
Fuerstenau, S, Benner, W H, Madden, N M, & Searles, W. Apparatus and method of determining molecular weight of large molecules. United States.
Fuerstenau, S, Benner, W H, Madden, N M, and Searles, W. Tue .
"Apparatus and method of determining molecular weight of large molecules". United States.
@article{osti_672527,
title = {Apparatus and method of determining molecular weight of large molecules},
author = {Fuerstenau, S and Benner, W H and Madden, N M and Searles, W},
abstractNote = {A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e{sup {minus}} are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation. 14 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 23 00:00:00 EDT 1998},
month = {Tue Jun 23 00:00:00 EDT 1998}
}