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Title: Apparatus and method of determining molecular weight of large molecules

Abstract

A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e.sup.- are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation.

Inventors:
 [1];  [2];  [3];  [4]
  1. (Montrose, CA)
  2. (Danville, CA)
  3. (Livermore, CA)
  4. (Fremont, CA)
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
OSTI Identifier:
871650
Patent Number(s):
5770857
Assignee:
Regents, University of California (Oakland, CA) LBNL
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
apparatus; method; determining; molecular; weight; molecules; mass; spectrometer; determines; multiply; charged; utilizes; detector; capable; simultaneously; measuring; charge; z; transit; time; single; passes; velocity; derived; providing; mass-to-charge; ratio; accelerated; potential; calculated; electrospray; masses; excess; mda; 425; readily; detected; on-axis; detection; configuration; enables; duty; cycle; nearly; 100; extends; practical; application; spectrometry; analysis; relatively; inexpensive; instrumentation; readily detected; simultaneously measuring; transit time; mass spectrometer; molecular weight; duty cycle; mass spectrometry; weight molecules; relatively inexpensive; spectrometer determines; determining molecular; /250/

Citation Formats

Fuerstenau, Stephen, Benner, W. Henry, Madden, Norman, and Searles, William. Apparatus and method of determining molecular weight of large molecules. United States: N. p., 1998. Web.
Fuerstenau, Stephen, Benner, W. Henry, Madden, Norman, & Searles, William. Apparatus and method of determining molecular weight of large molecules. United States.
Fuerstenau, Stephen, Benner, W. Henry, Madden, Norman, and Searles, William. Thu . "Apparatus and method of determining molecular weight of large molecules". United States. https://www.osti.gov/servlets/purl/871650.
@article{osti_871650,
title = {Apparatus and method of determining molecular weight of large molecules},
author = {Fuerstenau, Stephen and Benner, W. Henry and Madden, Norman and Searles, William},
abstractNote = {A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e.sup.- are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}

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