Recombinant yeast having increased tolerance to ionic liquids and methods of use
Abstract
The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae having enhanced tolerance for ionic liquid (IL) toxicity. Also provided are methods of using such genetically engineered yeast strains for improved IL-mediated hydrolysis of lignocellulosic biomass for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1998341
- Patent Number(s):
- 11639506
- Application Number:
- 15/154,537
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 05/13/2016
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Piotrowski, Jeff Scott, Bottoms, Scott, Dickinson, Quinn, and Landick, Robert Chase. Recombinant yeast having increased tolerance to ionic liquids and methods of use. United States: N. p., 2023.
Web.
Piotrowski, Jeff Scott, Bottoms, Scott, Dickinson, Quinn, & Landick, Robert Chase. Recombinant yeast having increased tolerance to ionic liquids and methods of use. United States.
Piotrowski, Jeff Scott, Bottoms, Scott, Dickinson, Quinn, and Landick, Robert Chase. Tue .
"Recombinant yeast having increased tolerance to ionic liquids and methods of use". United States. https://www.osti.gov/servlets/purl/1998341.
@article{osti_1998341,
title = {Recombinant yeast having increased tolerance to ionic liquids and methods of use},
author = {Piotrowski, Jeff Scott and Bottoms, Scott and Dickinson, Quinn and Landick, Robert Chase},
abstractNote = {The present invention relates to materials and methods for the production of ethanol. More particularly, the present invention provides genetically modified strains of Saccharomyces cerevisiae having enhanced tolerance for ionic liquid (IL) toxicity. Also provided are methods of using such genetically engineered yeast strains for improved IL-mediated hydrolysis of lignocellulosic biomass for industrial-scale production of various fuels, chemical feedstocks, and synthetic polymers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 02 00:00:00 EDT 2023},
month = {Tue May 02 00:00:00 EDT 2023}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast
journal, August 2006
- Parsons, Ainslie B.; Lopez, Andres; Givoni, Inmar E.
- Cell, Vol. 126, Issue 3
Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose
journal, August 2014
- Socha, A. M.; Parthasarathi, R.; Shi, J.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 35
The DNA Helicase, Hmi1p, Is Transported into Mitochondria by a C-terminal Cleavable Targeting Signal
journal, July 1999
- Lee, Connie M.; Sedman, Juhan; Neupert, Walter
- Journal of Biological Chemistry, Vol. 274, Issue 30
Genealogy of Principal Strains of the Yeast Genetic Stock Center
journal, May 1986
- Mortimer, Robert K.; Johnston, John R.
- Genetics, Vol. 113, Issue 1
Nonenzymatic Sugar Production from Biomass Using Biomass-Derived -Valerolactone
journal, January 2014
- Luterbacher, J. S.; Rand, J. M.; Alonso, D. M.
- Science, Vol. 343, Issue 6168
The STK2 gene, which encodes a putative Ser/Thr protein kinase, is required for high-affinity spermidine transport in Saccharomyces cerevisiae.
journal, June 1997
- Kaouass, M.; Audette, M.; Ramotar, D.
- Molecular and Cellular Biology, Vol. 17, Issue 6
The novel protein Ccz1p required for vacuolar assembly in Saccharomyces cerevisiae functions in the same transport pathway as Ypt7p
journal, December 2000
- Kucharczyk, Róza; Dupre, Sophie; Avaro, Sandrine
- Journal of Cell Science, Vol. 113, Issue 23
Regulation of Yeast H+-ATPase by Protein Kinases Belonging to a Family Dedicated to Activation of Plasma Membrane Transporters
journal, October 2000
- Goossens, A.; de la Fuente, N.; Forment, J.
- Molecular and Cellular Biology, Vol. 20, Issue 20
Engineering and Two-Stage Evolution of a Lignocellulosic Hydrolysate-Tolerant Saccharomyces cerevisiae Strain for Anaerobic Fermentation of Xylose from AFEX Pretreated Corn Stover
journal, September 2014
- Parreiras, Lucas S.; Breuer, Rebecca J.; Avanasi Narasimhan, Ragothaman
- PLoS ONE, Vol. 9, Issue 9
Impact of ionic liquid pretreated plant biomass on Saccharomyces cerevisiae growth and biofuel production
journal, January 2011
- Ouellet, Mario; Datta, Supratim; Dibble, Dean C.
- Green Chemistry, Vol. 13, Issue 10
Stress-related challenges in pentose fermentation to ethanol by the yeast Saccharomyces cerevisiae
journal, February 2011
- Almeida, João R. M.; Runquist, David; Sànchez Nogué, Violeta
- Biotechnology Journal, Vol. 6, Issue 3
Aromatic inhibitors derived from ammonia-pretreated lignocellulose hinder bacterial ethanologenesis by activating regulatory circuits controlling inhibitor efflux and detoxification
journal, August 2014
- Keating, David H.; Zhang, Yaoping; Ong, Irene M.
- Frontiers in Microbiology, Vol. 5
Understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations
journal, February 2012
- Taylor, Mark P.; Mulako, Inonge; Tuffin, Marla
- Biotechnology Journal, Vol. 7, Issue 9, p. 1169-1181
Harnessing Genetic Diversity in Saccharomyces cerevisiae for Fermentation of Xylose in Hydrolysates of Alkaline Hydrogen Peroxide-Pretreated Biomass
journal, November 2013
- Sato, Trey K.; Liu, Tongjun; Parreiras, Lucas S.
- Applied and Environmental Microbiology, Vol. 80, Issue 2
Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
journal, January 2007
- Almeida, Joao; Modig, Tobias; Petersson, Anneli
- Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349
Two genes of the putative mitochondrial fatty acid synthase in the genome of Saccharomyces cerevisiae
journal, December 1997
- Schneider, R.; Brors, Benedikt; Bürger, Frank
- Current Genetics, Vol. 32, Issue 6
Unbiased Screening of Marine Sponge Extracts for Anti-inflammatory Agents Combined with Chemical Genomics Identifies Girolline as an Inhibitor of Protein Synthesis
journal, October 2013
- Fung, Shan-Yu; Sofiyev, Vladimir; Schneiderman, Julia
- ACS Chemical Biology, Vol. 9, Issue 1
Scale-up and evaluation of high solid ionic liquid pretreatment and enzymatic hydrolysis of switchgrass
journal, January 2013
- Li, Chenlin; Tanjore, Deepti; He, Wei
- Biotechnology for Biofuels, Vol. 6, Issue 1
Molecular mechanisms of yeast tolerance and in situ detoxification of lignocellulose hydrolysates
journal, March 2011
- Liu, Z. Lewis
- Applied Microbiology and Biotechnology, Vol. 90, Issue 3
Advances and Developments in Strategies to Improve Strains of Saccharomyces cerevisiae and Processes to Obtain the Lignocellulosic Ethanol−A Review
journal, March 2012
- Laluce, C.; Schenberg, A. C. G.; Gallardo, J. C. M.
- Applied Biochemistry and Biotechnology, Vol. 166, Issue 8
Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors
journal, March 2014
- Piotrowski, Jeff S.; Zhang, Yaoping; Bates, Donna M.
- Frontiers in Microbiology, Vol. 5
Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain
journal, January 2016
- Dickinson, Quinn; Bottoms, Scott; Hinchman, Li
- Microbial Cell Factories, Vol. 15, Issue 1
A New Yeast Metabolon Involving at Least the Two First Enzymes of Arginine Biosynthesis
journal, November 2001
- Abadjieva, Agnes; Pauwels, Katia; Hilven, Pierre
- Journal of Biological Chemistry, Vol. 276, Issue 46
Integrated Catalytic Conversion of γ-Valerolactone to Liquid Alkenes for Transportation Fuels
journal, February 2010
- Bond, J. Q.; Alonso, D. M.; Wang, D.
- Science, Vol. 327, Issue 5969, p. 1110-1114
Transposon Mutagenesis To Improve the Growth of Recombinant Saccharomyces cerevisiae on D-Xylose
journal, February 2007
- Ni, H.; Laplaza, J. M.; Jeffries, T. W.
- Applied and Environmental Microbiology, Vol. 73, Issue 7
Glucose repression on RIM1 , a gene encoding a mitochondrial single-stranded DNA-binding protein, in Saccharomyces cerevisiae : a possible regulation at pre-mRNA splicing
journal, December 1998
- Li, Zhijun; Ling, F.; Shibata, T.
- Current Genetics, Vol. 34, Issue 5
Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase.
journal, January 1995
- Walfridsson, M.; Hallborn, J.; Penttilä, M.
- Applied and environmental microbiology, Vol. 61, Issue 12
Deletions of SKY1 or PTK2 in the Saccharomyces cerevisiaetrk1Δtrk2Δ mutant cells exert dual effect on ion homeostasis
journal, August 2002
- Erez, Omri; Kahana, Chaim
- Biochemical and Biophysical Research Communications, Vol. 295, Issue 5
Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
- Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
- Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition
journal, August 2000
- Palmqvist, Eva; Hahn-Hägerdal, Bärbel
- Bioresource Technology, Vol. 74, Issue 1, p. 25-33
Saccharomyces Cerevisiae Strains
patent-application, November 2013
- Albers, Eva; Olsson, Lisbeth; Koppram, Rakesh
- US Patent Application 13/885031; 20130295620
Screening for Modulators of Spermine Tolerance Identifies Sky1, the SR Protein Kinase of Saccharomyces cerevisiae, as a Regulator of Polyamine Transport and Ion Homeostasis
journal, January 2001
- Erez, O.; Kahana, C.
- Molecular and Cellular Biology, Vol. 21, Issue 1
Yeast protein kinase Ptk2 localizes at the plasma membrane and phosphorylates in vitro the C-terminal peptide of the H+-ATPase
journal, February 2006
- Eraso, Pilar; Mazón, María J.; Portillo, Francisco
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 2
Toxicity and antimicrobial activity of imidazolium and pyridinium ionic liquids
journal, January 2005
- Docherty, Kathryn M.; Kulpa, Jr., Charles F.
- Green Chemistry, Vol. 7, Issue 4