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Title: Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1416354
Assignee:
The Regents of the University of California (Oakland, CA); Sandia Corporation (Albuquerque, NM) LBNL
Patent Number(s):
9,862,982
Application Number:
15/164,813
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2016 May 25
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

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