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Title: Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases

Abstract

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1416354
Patent Number(s):
9862982
Application Number:
15/164,813
Assignee:
The Regents of the University of California (Oakland, CA); Sandia Corporation (Albuquerque, NM)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 May 25
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M. Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases. United States: N. p., 2018. Web.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., & Rubin, Edward M. Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases. United States.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M. Tue . "Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases". United States. https://www.osti.gov/servlets/purl/1416354.
@article{osti_1416354,
title = {Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases},
author = {Zhang, Tao and Datta, Supratim and Simmons, Blake A. and Rubin, Edward M.},
abstractNote = {The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {1}
}

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