Title: Optical device for in-line and real-time monitoring of microorganisms

Abstract

A multi-wavelength laser diode based optical sensor system capable of monitoring the dynamics and physiological changes of a microorganism culture in real-time. The microorganism culture from a microorganism production chamber is pumped to a flow chamber. Laser diodes emit light at certain wavelengths through the flow chamber, which is sensed by photodiodes. A laser control circuitry is operatively connected to the laser diodes and a signal conditioning circuitry is operatively connected to the photodiodes. A microprocessor reads and records voltage signals corresponding to the wavelengths. A data acquisition system converts said voltage signals into measurements of biological parameters, which are displayed on a graphical user interface and allow a user to monitor the measurements in real time.

Inventors:

Kacira, Murat; Jia, Fei; Ogden, Kimberly L.; Ogden, Gregory E.

Issue Date:

Tue Mar 01 00:00:00 EST 2022

Research Org.:

Univ. of Arizona, Tucson, AZ (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1892660

Patent Number(s):

11261474

Application Number:

15/739,467

Assignee:

Arizona Board of Regents on Behalf of the University of Arizona (Tucson, AZ)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY

C12M21/02 - {Photobioreactors
C12M23/04 - {Flat or tray type, drawers
C12M23/18 - {Open ponds
C12M29/08 - {Air lift}
C12M41/06 - {of illumination}
C12M41/36 - {of biomass, e.g. colony counters or by turbidity measurements

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/02 - involving viable microorganisms

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DOE Contract Number:  

EE0006269

Resource Type:

Patent

Resource Relation:

Patent File Date: 06/29/2016

Country of Publication:

United States

Language:

English

Subject:

47 OTHER INSTRUMENTATION