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Title: Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms

Abstract

In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.

Inventors:
 [1];  [2];  [3];  [4]
  1. Lakewood, CO
  2. Golden, CO
  3. Pushchino, RU
  4. Moscow, RU
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
909588
Patent Number(s):
7229785
Application Number:
10/511,929
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Seibert, Michael, Makarova, Valeriya, Tsygankov, Anatoly A, and Rubin, Andrew B. Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms. United States: N. p., 2007. Web.
Seibert, Michael, Makarova, Valeriya, Tsygankov, Anatoly A, & Rubin, Andrew B. Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms. United States.
Seibert, Michael, Makarova, Valeriya, Tsygankov, Anatoly A, and Rubin, Andrew B. Tue . "Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms". United States. https://www.osti.gov/servlets/purl/909588.
@article{osti_909588,
title = {Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms},
author = {Seibert, Michael and Makarova, Valeriya and Tsygankov, Anatoly A and Rubin, Andrew B},
abstractNote = {In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {6}
}

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