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Title: Methods for threshold determination in multiplexed assays

Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.
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Issue Date:
OSTI Identifier:
Lawrence Livermore National Security, LLC (Livermore, CA) LLNL
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2009 Oct 28
Research Org:
Univ. of California, Berkeley, CA (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Comparing Some Operational Approaches to the Limit of Detection
journal, July 2004
  • Desimoni, Elio; Brunetti, Barbara; Cattaneo, Raffaella
  • Annali di Chimica, Vol. 94, Issue 78, p. 555-569
  • DOI: 10.1002/adic.200490069

A review of foot-and-mouth disease with special consideration for the clinical and epidemiological factors relevant to predictive modelling of the disease
journal, March 2005
  • Kitching, R. P.; Hutber, A. M.; Thrusfield, M. V.
  • The Veterinary Journal, Vol. 169, Issue 2, p. 197-209
  • DOI: 10.1016/j.tvjl.2004.06.001

Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT-PCR assay
journal, August 2003

Development of a novel real-time RT-PCR assay for quantitation of foot-and-mouth disease virus in diverse porcine tissues
journal, March 2001
  • Oleksiewicz, Martin B.; Donaldson, Alex I.; Alexandersen, Soren
  • Journal of Virological Methods, Vol. 92, Issue 1, p. 23-35
  • DOI: 10.1016/S0166-0934(00)00265-2

Fluorogenic RT–PCR assay (TaqMan) for detection and classification of bovine viral diarrhea virus
journal, October 2001

A one-step multiplex real-time RT-PCR for detection and typing of bovine viral diarrhea viruses
journal, August 2006

Validation of a reverse transcriptase multiplex PCR test for the serotype determination of U.S. isolates of bluetongue virus
journal, September 2000

A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents
journal, April 2005
  • Wilson, Wendy J.; Erler, Anne M.; Nasarabadi, Shanavaz L.
  • Molecular and Cellular Probes, Vol. 19, Issue 2, p. 137-144
  • DOI: 10.1016/j.mcp.2004.10.005

Applications of Luminex® xMAP™ technology for rapid, high-throughput multiplexed nucleic acid detection
journal, January 2006

Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests
journal, May 2000

A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests
journal, September 1995
  • Greiner, Matthias; Sohr, Dorit; Göbel, Petra
  • Journal of Immunological Methods, Vol. 185, Issue 1, p. 123-132
  • DOI: 10.1016/0022-1759(95)00121-P

Quantitative, multiplexed detection of bacterial pathogens: DNA and protein applications of the Luminex LabMAP™ system
journal, May 2003
  • Dunbar, Sherry A.; Vander Zee, Coe A.; Oliver, Kerry G.
  • Journal of Microbiological Methods, Vol. 53, Issue 2, p. 245-252
  • DOI: 10.1016/S0167-7012(03)00028-9

Development of a real-time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus Vesivirus)
journal, March 2007
  • Reid, Scott M.; King, Donald P.; Shaw, Andrew E.
  • Journal of Virological Methods, Vol. 140, Issue 1-2, p. 166-173
  • DOI: 10.1016/j.jviromet.2006.11.010