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Title: Methods for threshold determination in multiplexed assays

Abstract

Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of California, Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1134715
Patent Number(s):
8,762,068
Application Number:
12/607,956
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Oct 28
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. Methods for threshold determination in multiplexed assays. United States: N. p., 2014. Web.
Tammero, Lance F. Bentley, Dzenitis, John M, & Hindson, Benjamin J. Methods for threshold determination in multiplexed assays. United States.
Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. Tue . "Methods for threshold determination in multiplexed assays". United States. https://www.osti.gov/servlets/purl/1134715.
@article{osti_1134715,
title = {Methods for threshold determination in multiplexed assays},
author = {Tammero, Lance F. Bentley and Dzenitis, John M and Hindson, Benjamin J},
abstractNote = {Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {6}
}

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