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Title: Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same

Abstract

A microfabricated device and method for electrokinetic transport of a liquid phase biological or chemical material is described. In accordance with one aspect of the present invention there is provided a microchip that is adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on a substrate. The apparatus includes a focusing chamber formed in a surface of the substrate and in fluid communication with two sample fluid channels and three focusing fluid channels. The device further includes electromotive means operatively connected to the sources of the sample fluid and the source of focusing fluid for electrokinetically driving the respective streams of the sample and focusing fluids through the respective channels into the focusing chamber such that the focusing fluid streams spatially confine the first and second sample fluid streams within the focusing chamber. In accordance with another aspect of this invention, there is provided a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In the disclosed cytometry process the width of the fluid stream is narrowed in the focusing chamber. The microscopic particles in the focused sample fluid are then detected and/or measured usingmore » light scattering or other techniques.« less

Inventors:
 [1];  [1]
  1. (Knoxville, TN)
Issue Date:
Research Org.:
LOCKHEED MARTIN ENERGY RES COR
OSTI Identifier:
873247
Patent Number(s):
6120666
Assignee:
UT-Battelle, LLC (Oak Ridge, TN) ORNL
DOE Contract Number:  
AC05-96OR22464
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
microfabricated; device; method; multiplexed; electrokinetic; focusing; fluid; streams; transport; cytometry; liquid; phase; biological; chemical; material; described; accordance; aspect; provided; microchip; adapted; simultaneous; spatial; confinement; electrokinetically; driven; fluidic; substrate; apparatus; chamber; formed; surface; communication; sample; channels; electromotive; means; operatively; connected; sources; source; driving; respective; fluids; spatially; confine; analyzing; microscopic; particles; medium; utilizing; function; disclosed; process; width; stream; narrowed; focused; detected; measured; light; scattering; techniques; microfabricated device; fluid streams; microscopic particles; light scattering; fluid stream; liquid phase; fluid communication; sample fluid; operatively connected; fluid medium; chamber formed; fluid channels; chemical material; electrokinetic transport; means operative; analyzing micro; /204/137/422/435/

Citation Formats

Jacobson, Stephen C., and Ramsey, J. Michael. Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same. United States: N. p., 2000. Web.
Jacobson, Stephen C., & Ramsey, J. Michael. Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same. United States.
Jacobson, Stephen C., and Ramsey, J. Michael. Sat . "Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same". United States. https://www.osti.gov/servlets/purl/873247.
@article{osti_873247,
title = {Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same},
author = {Jacobson, Stephen C. and Ramsey, J. Michael},
abstractNote = {A microfabricated device and method for electrokinetic transport of a liquid phase biological or chemical material is described. In accordance with one aspect of the present invention there is provided a microchip that is adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on a substrate. The apparatus includes a focusing chamber formed in a surface of the substrate and in fluid communication with two sample fluid channels and three focusing fluid channels. The device further includes electromotive means operatively connected to the sources of the sample fluid and the source of focusing fluid for electrokinetically driving the respective streams of the sample and focusing fluids through the respective channels into the focusing chamber such that the focusing fluid streams spatially confine the first and second sample fluid streams within the focusing chamber. In accordance with another aspect of this invention, there is provided a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In the disclosed cytometry process the width of the fluid stream is narrowed in the focusing chamber. The microscopic particles in the focused sample fluid are then detected and/or measured using light scattering or other techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

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