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Title: Data transformation methods for multiplexed assays

Abstract

Methods to improve the performance of an array assay are described. A correlation between fluorescence intensity-related parameters and negative control values of the assay is determined. The parameters are then adjusted as a function of the correlation. As a result, sensitivity of the assay is improved without changes in its specificity.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1088677
Patent Number(s):
8494780
Application Number:
12/698,785
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
97 MATHEMATICS AND COMPUTING

Citation Formats

Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. Data transformation methods for multiplexed assays. United States: N. p., 2013. Web.
Tammero, Lance F. Bentley, Dzenitis, John M, & Hindson, Benjamin J. Data transformation methods for multiplexed assays. United States.
Tammero, Lance F. Bentley, Dzenitis, John M, and Hindson, Benjamin J. Tue . "Data transformation methods for multiplexed assays". United States. https://www.osti.gov/servlets/purl/1088677.
@article{osti_1088677,
title = {Data transformation methods for multiplexed assays},
author = {Tammero, Lance F. Bentley and Dzenitis, John M and Hindson, Benjamin J},
abstractNote = {Methods to improve the performance of an array assay are described. A correlation between fluorescence intensity-related parameters and negative control values of the assay is determined. The parameters are then adjusted as a function of the correlation. As a result, sensitivity of the assay is improved without changes in its specificity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {7}
}

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