Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency
Abstract
Devices for measuring single cell changes in photosynthetic efficiency in algal aquaculture are disclosed that include a combination of modulated LED trans-illumination of different intensities with synchronized through objective laser illumination and confocal detection. Synchronization and intensity modulation of a dual illumination scheme were provided using a custom microcontroller for a laser beam block and constant current LED driver. Therefore, single whole cell photosynthetic efficiency, and subcellular (diffraction limited) photosynthetic efficiency measurement modes are permitted. Wide field rapid light scanning actinic illumination is provided for both by an intensity modulated 470 nm LED. For the whole cell photosynthetic efficiency measurement, the same LED provides saturating pulses for generating photosynthetic induction curves. For the subcellular photosynthetic efficiency measurement, a switched through objective 488 nm laser provides saturating pulses for generating photosynthetic induction curves. A second near IR LED is employed to generate dark adapted states in the system under study.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1088291
- Patent Number(s):
- 8481974
- Application Number:
- 13/233,343
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING
Citation Formats
Davis, Ryan Wesley, Singh, Seema, and Wu, Huawen. Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency. United States: N. p., 2013.
Web.
Davis, Ryan Wesley, Singh, Seema, & Wu, Huawen. Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency. United States.
Davis, Ryan Wesley, Singh, Seema, and Wu, Huawen. Tue .
"Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency". United States. https://www.osti.gov/servlets/purl/1088291.
@article{osti_1088291,
title = {Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency},
author = {Davis, Ryan Wesley and Singh, Seema and Wu, Huawen},
abstractNote = {Devices for measuring single cell changes in photosynthetic efficiency in algal aquaculture are disclosed that include a combination of modulated LED trans-illumination of different intensities with synchronized through objective laser illumination and confocal detection. Synchronization and intensity modulation of a dual illumination scheme were provided using a custom microcontroller for a laser beam block and constant current LED driver. Therefore, single whole cell photosynthetic efficiency, and subcellular (diffraction limited) photosynthetic efficiency measurement modes are permitted. Wide field rapid light scanning actinic illumination is provided for both by an intensity modulated 470 nm LED. For the whole cell photosynthetic efficiency measurement, the same LED provides saturating pulses for generating photosynthetic induction curves. For the subcellular photosynthetic efficiency measurement, a switched through objective 488 nm laser provides saturating pulses for generating photosynthetic induction curves. A second near IR LED is employed to generate dark adapted states in the system under study.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 09 00:00:00 EDT 2013},
month = {Tue Jul 09 00:00:00 EDT 2013}
}
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