skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components

Abstract

Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Security LLC, Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568650
Patent Number(s):
10,365,220
Application Number:
11/916,273
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
PHY-012099; W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 05/31/2006
Country of Publication:
United States
Language:
English

Citation Formats

Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, and Li, Ronald. Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components. United States: N. p., 2019. Web.
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, & Li, Ronald. Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components. United States.
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, and Li, Ronald. Tue . "Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components". United States. https://www.osti.gov/servlets/purl/1568650.
@article{osti_1568650,
title = {Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components},
author = {Chan, James W. and Huser, Thomas R. and Lane, Stephen M. and Rutledge, John C. and Taylor, Douglas S. and Zwerdling, Theodore and Li, Ronald},
abstractNote = {Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {7}
}

Patent:

Save / Share: