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Title: Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components

Abstract

Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568650
Patent Number(s):
10365220
Application Number:
11/916,273
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
PHY-012099; W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 05/31/2006
Country of Publication:
United States
Language:
English

Citation Formats

Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, and Li, Ronald. Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components. United States: N. p., 2019. Web.
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, & Li, Ronald. Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components. United States.
Chan, James W., Huser, Thomas R., Lane, Stephen M., Rutledge, John C., Taylor, Douglas S., Zwerdling, Theodore, and Li, Ronald. Tue . "Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components". United States. https://www.osti.gov/servlets/purl/1568650.
@article{osti_1568650,
title = {Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components},
author = {Chan, James W. and Huser, Thomas R. and Lane, Stephen M. and Rutledge, John C. and Taylor, Douglas S. and Zwerdling, Theodore and Li, Ronald},
abstractNote = {Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {7}
}

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Works referenced in this record:

Near-infrared raman spectroscopy for in vitro and in vivo detection of cervical precancers
patent, November 1999


Method for adjusting spectral measurements to produce a standard Raman spectrum
patent, August 2001


Method for sorting particles
patent, December 2004


Apparatus and method for immobilizing molecules onto a substrate
patent, October 2000


Optical stretcher
patent, May 2000


Non-destructive optical trap for biological particles and method of doing same
patent, January 1990