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Title: Methods and compounds for chemical ligation

Abstract

Compositions and methods for chemical ligation are provided. Methods for nucleic acid sequencing, nucleic acid assembly and nucleic acid synthesis are also provided.

Inventors:
;
Issue Date:
Research Org.:
Harvard College (Cambridge, MA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1087892
Patent Number(s):
8481258
Application Number:
12/628,518
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Church, George M., and Sismour, A. Michael. Methods and compounds for chemical ligation. United States: N. p., 2013. Web.
Church, George M., & Sismour, A. Michael. Methods and compounds for chemical ligation. United States.
Church, George M., and Sismour, A. Michael. Tue . "Methods and compounds for chemical ligation". United States. https://www.osti.gov/servlets/purl/1087892.
@article{osti_1087892,
title = {Methods and compounds for chemical ligation},
author = {Church, George M. and Sismour, A. Michael},
abstractNote = {Compositions and methods for chemical ligation are provided. Methods for nucleic acid sequencing, nucleic acid assembly and nucleic acid synthesis are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {7}
}

Works referenced in this record:

SNPs analysis by use of oligonucleotides containing 2′-O-methyl-2-thiouridines
journal, September 2005


In vitro selection of optimal DNA substrates for ligation by a water-soluble carbodiimide
journal, June 1994


Unambiguous demonstration of triple-helix-directed gene modification
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Chemical ligation as a method for the assembly of double-stranded nucleic acids: Modifications and local structure studies
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The use of BrCN for assembling modified DNA duplexes and DNA-RNA hybrids; comparison with water-soluble carbodiimide
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Advanced sequencing technologies: methods and goals
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Kinetic model studies on the chemical ligation of oligonucleotides via hydrazone formation
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Use of a new retrieving adaptor in the cloning of a synthetic human insulin A-chain gene
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Template-Directed Assembly and Characterization of Metallosalen−DNA Conjugates
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Site-directed modification of DNA duplexes by chemical ligation
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Chemical reactions within DNA duplexes Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent
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Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays
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Oligonucleotide circularization by template-directed chemical ligation
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Chemical ligation of oligodeoxyribonucleotides on circular DNA templates
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Oligonucleotide N3'-->P5' phosphoramidates as antisense agents
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Template-directed synthesis: use of a reversible reaction
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Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
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Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene
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