skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods for detection of methyl-CpG dinucleotides

Abstract

The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5'-C.sup.MeCpGG-3'. Methods for making and using the rMcrA protein, and derivatives thereof are provided.

Inventors:
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1078331
Patent Number(s):
8263742
Application Number:
13/085,219
Assignee:
Brookhaven Science Associates, LLC (Upton, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dunn, John J. Methods for detection of methyl-CpG dinucleotides. United States: N. p., 2012. Web.
Dunn, John J. Methods for detection of methyl-CpG dinucleotides. United States.
Dunn, John J. Tue . "Methods for detection of methyl-CpG dinucleotides". United States. https://www.osti.gov/servlets/purl/1078331.
@article{osti_1078331,
title = {Methods for detection of methyl-CpG dinucleotides},
author = {Dunn, John J.},
abstractNote = {The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5'-C.sup.MeCpGG-3'. Methods for making and using the rMcrA protein, and derivatives thereof are provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {9}
}

Patent:

Save / Share:

Works referenced in this record:

Evidence for an instructive mechanism of de novo methylation in cancer cells
journal, January 2006


MethyLight: a high-throughput assay to measure DNA methylation
journal, April 2000


Genome-Wide Profiling of CpG Methylation Identifies Novel Targets of Aberrant Hypermethylation in Myeloid Leukemia
journal, June 2006


McrA and McrB restriction phenotypes of some E.coli strains and implications for gene cloning
journal, January 1988


Direct Labeling of 5-Methylcytosine and Its Applications
journal, May 2007


Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells
journal, July 2005


MeCP2 Binding to DNA Depends upon Hydration at Methyl-CpG
journal, February 2008


Molecular cloning and sequencing of mcrA locus and identification of McrA protein inEscherichia coli
journal, September 1992


LUMA (LUminometric Methylation Assay)—A high throughput method to the analysis of genomic DNA methylation
journal, July 2006


COBRA: a sensitive and quantitative DNA methylation assay
journal, June 1997


A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.
journal, March 1992


Purification of CpG islands using a methylated DNA binding column
journal, March 1994


Rapid and sensitive detection of CpG-methylation using methyl-binding (MB)-PCR
journal, June 2006


High sensitivity mapping of methylated cytosines
journal, January 1994


Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
journal, September 1996