skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methyl-CpG island-associated genome signature tags

Abstract

Disclosed is a method for analyzing the organismic complexity of a sample through analysis of the nucleic acid in the sample. In the disclosed method, through a series of steps, including digestion with a type II restriction enzyme, ligation of capture adapters and linkers and digestion with a type IIS restriction enzyme, genome signature tags are produced. The sequences of a statistically significant number of the signature tags are determined and the sequences are used to identify and quantify the organisms in the sample. Various embodiments of the invention described herein include methods for using single point genome signature tags to analyze the related families present in a sample, methods for analyzing sequences associated with hyper- and hypo-methylated CpG islands, methods for visualizing organismic complexity change in a sampling location over time and methods for generating the genome signature tag profile of a sample of fragmented DNA.

Inventors:
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1132262
Patent Number(s):
8,728,733
Application Number:
11/926,203
Assignee:
Brookhaven Science Associates, LLC (Upton, NY)
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Oct 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dunn, John J. Methyl-CpG island-associated genome signature tags. United States: N. p., 2014. Web.
Dunn, John J. Methyl-CpG island-associated genome signature tags. United States.
Dunn, John J. Tue . "Methyl-CpG island-associated genome signature tags". United States. https://www.osti.gov/servlets/purl/1132262.
@article{osti_1132262,
title = {Methyl-CpG island-associated genome signature tags},
author = {Dunn, John J},
abstractNote = {Disclosed is a method for analyzing the organismic complexity of a sample through analysis of the nucleic acid in the sample. In the disclosed method, through a series of steps, including digestion with a type II restriction enzyme, ligation of capture adapters and linkers and digestion with a type IIS restriction enzyme, genome signature tags are produced. The sequences of a statistically significant number of the signature tags are determined and the sequences are used to identify and quantify the organisms in the sample. Various embodiments of the invention described herein include methods for using single point genome signature tags to analyze the related families present in a sample, methods for analyzing sequences associated with hyper- and hypo-methylated CpG islands, methods for visualizing organismic complexity change in a sampling location over time and methods for generating the genome signature tag profile of a sample of fragmented DNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record:

Genomic Signature Tags (GSTs): A System for Profiling Genomic DNA
journal, November 2002


Defining the CREB Regulon: A Genome-Wide Analysis of Transcription Factor Regulatory Regions
journal, December 2004


Genome-Wide Location and Function of DNA Binding Proteins
journal, December 2000


Comprehensive analysis of CpG islands in human chromosomes 21 and 22
journal, March 2002

  • Takai, Daiya; Jones, Peter A.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 6, p. 3740-3745
  • DOI: 10.1073/pnas.052410099