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Title: Methyl-CpG island-associated genome signature tags

Disclosed is a method for analyzing the organismic complexity of a sample through analysis of the nucleic acid in the sample. In the disclosed method, through a series of steps, including digestion with a type II restriction enzyme, ligation of capture adapters and linkers and digestion with a type IIS restriction enzyme, genome signature tags are produced. The sequences of a statistically significant number of the signature tags are determined and the sequences are used to identify and quantify the organisms in the sample. Various embodiments of the invention described herein include methods for using single point genome signature tags to analyze the related families present in a sample, methods for analyzing sequences associated with hyper- and hypo-methylated CpG islands, methods for visualizing organismic complexity change in a sampling location over time and methods for generating the genome signature tag profile of a sample of fragmented DNA.
Inventors:
Issue Date:
OSTI Identifier:
1132262
Assignee:
Brookhaven Science Associates, LLC (Upton, NY) BNL
Patent Number(s):
8,728,733
Application Number:
11/926,203
Contract Number:
AC02-98CH10886
Resource Relation:
Patent File Date: 2007 Oct 29
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Defining the CREB Regulon: A Genome-Wide Analysis of Transcription Factor Regulatory Regions
journal, December 2004

Comprehensive analysis of CpG islands in human chromosomes 21 and 22
journal, March 2002
  • Takai, Daiya; Jones, Peter A.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 6, p. 3740-3745
  • DOI: 10.1073/pnas.052410099

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