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Title: Nested methylation-specific polymerase chain reaction cancer detection method

Abstract

A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.

Inventors:
 [1];  [2]
  1. Albuquerque, NM
  2. Edgewood, NM
Issue Date:
Research Org.:
Lovelace Respiratory Research Institute (Albuquerque, NM); St Mary's Hospital and Medical Center
Sponsoring Org.:
USDOE
OSTI Identifier:
909343
Patent Number(s):
7214485
Application Number:
10/344,815
Assignee:
Lovelace Respiratory Research Institute (Albuquerque, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FC04-96AL76406; FG02-90ER60939
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Belinsky, Steven A, and Palmisano, William A. Nested methylation-specific polymerase chain reaction cancer detection method. United States: N. p., 2007. Web.
Belinsky, Steven A, & Palmisano, William A. Nested methylation-specific polymerase chain reaction cancer detection method. United States.
Belinsky, Steven A, and Palmisano, William A. Tue . "Nested methylation-specific polymerase chain reaction cancer detection method". United States. https://www.osti.gov/servlets/purl/909343.
@article{osti_909343,
title = {Nested methylation-specific polymerase chain reaction cancer detection method},
author = {Belinsky, Steven A and Palmisano, William A},
abstractNote = {A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {5}
}

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Works referenced in this record:

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