Using DNA Affinity Purification sequencing (DAP-seq) to identify in vitro binding sites of transcription factors potentially involved in aromatic degradation

Donohue, Timothy J.; Noguera, Daniel R.

doi:10.82250/3003637

Title: Using DNA Affinity Purification sequencing (DAP-seq) to identify in vitro binding sites of transcription factors potentially involved in aromatic degradation

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Abstract

The genome-wide binding sites of 44 transcription factors from the aromatic metabolizing Alphaproteobacterium Novosphingobium aromaticivorans were identified using DNA Affinity Purification sequencing (DAP-seq). We report 32 of these transcription factors have at least one area of enrichment. These data will be valuable for better understanding of aromatic metabolism.

Authors:

Donohue, Timothy J.; Noguera, Daniel R.

GLBRC - University of Wisconsin

Publication Date:: Wed Mar 12 04:00:00 UTC 2025

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: DAP-seq; aromatic metabolism; bacteria; transcription factor

OSTI Identifier:: 3003637

DOI:: https://doi.org/10.82250/3003637

Citation Formats


                    Donohue, Timothy J., and Noguera, Daniel R. Using DNA Affinity Purification sequencing (DAP-seq) to identify in vitro binding sites of transcription factors potentially involved in aromatic degradation.  United States: N. p., 2025. 
        Web.  doi:10.82250/3003637.

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                    Donohue, Timothy J., & Noguera, Daniel R. Using DNA Affinity Purification sequencing (DAP-seq) to identify in vitro binding sites of transcription factors potentially involved in aromatic degradation.  United States.  doi:https://doi.org/10.82250/3003637

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                    Donohue, Timothy J., and Noguera, Daniel R. 2025.  
"Using DNA Affinity Purification sequencing (DAP-seq) to identify in vitro binding sites of transcription factors potentially involved in aromatic degradation".  United States.  doi:https://doi.org/10.82250/3003637.  https://www.osti.gov/servlets/purl/3003637. Pub date:Wed Mar 12 04:00:00 UTC 2025

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@article{osti_3003637,

  title        = {Using DNA Affinity Purification sequencing (DAP-seq) to identify in vitro binding sites of transcription factors potentially involved in aromatic degradation},

  author       = {Donohue, Timothy J. and Noguera, Daniel R.},

  abstractNote = {The genome-wide binding sites of 44 transcription factors from the aromatic metabolizing Alphaproteobacterium Novosphingobium aromaticivorans were identified using DNA Affinity Purification sequencing (DAP-seq). We report 32 of these transcription factors have at least one area of enrichment. These data will be valuable for better understanding of aromatic metabolism.},

  doi          = {10.82250/3003637},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Mar 12 04:00:00 UTC 2025},

  month        = {Wed Mar 12 04:00:00 UTC 2025}

}

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DOI: https://doi.org/10.82250/3003637

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Using DNA affinity purification sequencing (DAP-seq) to identify in vitro binding sites of potential Novosphingobium aromaticivorans DSM12444 transcription factors

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