Using DNA affinity purification sequencing (DAP-seq) to identify in vitro binding sites of potential Novosphingobium aromaticivorans DSM12444 transcription factors

Myers, Kevin S.; Linz, Alexandra M.; Baumgart, Leo A.; Zhang, Yu; Blaby, Ian; Mewalal, Ritesh; Ng, Vivian; Noguera, Daniel R.; Donohue, Timothy J.

doi:10.1128/mra.00260-25

Using DNA affinity purification sequencing (DAP-seq) to identify in vitro binding sites of potential Novosphingobium aromaticivorans DSM12444 transcription factors

Journal Article · Tue Jul 22 00:00:00 EDT 2025 · Microbiology Resource Announcements

DOI:https://doi.org/10.1128/mra.00260-25· OSTI ID:2573971

^[1]; Linz, Alexandra M. ^[1]; Baumgart, Leo A. ^[2]; Zhang, Yu ^[2]; Blaby, Ian ^[2]; Mewalal, Ritesh ^[2]; Ng, Vivian ^[2]; ^[1]; ^[1]

University of Wisconsin-Madison, WI (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Genome-wide binding sites of 44 putative transcription factors (TFs) from Novosphingobium aromaticivorans DSM12444 were analyzed using DNA affinity purification sequencing. We report that 32 of these TFs have at least one area of enrichment. These data will help better understand aromatic metabolism and other features of N. aromaticivorans biology.

View Accepted Manuscript (DOE)

Research Organization:: University of Wisconsin-Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409; AC02-05CH11231

OSTI ID:: 2573971

Journal Information:: Microbiology Resource Announcements, Journal Name: Microbiology Resource Announcements; ISSN 2576-098X

Publisher:: SSPA - American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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