The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks

Mullet, John

doi:10.82250/3003569

Title: The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks

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Abstract

Stems accumulate high levels of sucrose that could be used to synthesize bioethanol and useful biopolymers if information about stem cell-type gene expression and regulation was available to enable engineering. To obtain this information, Laser Capture Microdissection (LCM) was used to isolate and collect transcriptome profiles from five major cell types that are present in stems of the sweet sorghum Wray. Transcriptome analysis identified genes with cell-type specific and cell-preferred expression patterns that reflect the distinct metabolic, transport, and regulatory functions of each cell type.

Authors:

Mullet, John

GLBRC - Texas A&M University

Publication Date:: Thu Feb 08 23:00:00 EST 2024

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: cell specificity; gene regulatory network; laser capture microdissection; secondary cell wall; sorghum; stem

OSTI Identifier:: 3003569

DOI:: https://doi.org/10.82250/3003569

Citation Formats


                    Mullet, John. The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks  .  United States: N. p., 2024. 
        Web.  doi:10.82250/3003569.

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                    Mullet, John. The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks  .  United States.  doi:https://doi.org/10.82250/3003569

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                    Mullet, John. 2024.  
"The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks  ".  United States.  doi:https://doi.org/10.82250/3003569.  https://www.osti.gov/servlets/purl/3003569. Pub date:Thu Feb 08 23:00:00 EST 2024

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@article{osti_3003569,

  title        = {The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks  },

  author       = {Mullet, John},

  abstractNote = {Stems accumulate high levels of sucrose that could be used to synthesize bioethanol and useful biopolymers if information about stem cell-type gene expression and regulation was available to enable engineering. To obtain this information, Laser Capture Microdissection (LCM) was used to isolate and collect transcriptome profiles from five major cell types that are present in stems of the sweet sorghum Wray. Transcriptome analysis identified genes with cell-type specific and cell-preferred expression patterns that reflect the distinct metabolic, transport, and regulatory functions of each cell type.},

  doi          = {10.82250/3003569},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Feb 08 23:00:00 EST 2024},

  month        = {Thu Feb 08 23:00:00 EST 2024}

}

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DOI: https://doi.org/10.82250/3003569

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