Pichia sp. yHMH446 translation elongation factor 1-alpha gene, partial cds

Opulente, Dana A.; Hittinger, Chris Todd

doi:10.82250/3003519

Title: Pichia sp. yHMH446 translation elongation factor 1-alpha gene, partial cds

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Abstract

A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genus Pichia. Strain yHMH446 falls within a clade that includes Pichia norvegensis, Pichia pseudocactophila, Candida inconspicua, and Pichia cactophila. Whole genome sequence data were analyzed for the presence of genes known to be important for carbon and nitrogen metabolism, and the phenotypic data from the novel species were compared to all Pichia species with publicly available genomes. Across the genus, including the novel species candidate, we found that the inability to use many carbon and nitrogen sources correlated with the absence of metabolic genes. Based on these results, Pichia galeolata sp. nov. is proposed to accommodate yHMH446(T) (=NRRL Y-64187 = CBS 16864). This study shows how integrated taxogenomic analysis can add mechanistic insight to species descriptions.

Authors:

Opulente, Dana A.; Hittinger, Chris Todd

Biology Department Villanova University, Villanova, Pennsylvania, USA
GLBRC - University of Wisconsin

Publication Date:: Wed Jan 19 04:00:00 UTC 2022

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: genome sequence; genotype-phenotype map; novel species; taxogenomics; yeast

OSTI Identifier:: 3003519

DOI:: https://doi.org/10.82250/3003519

Citation Formats


                    Opulente, Dana A., and Hittinger, Chris Todd. Pichia sp. yHMH446 translation elongation factor 1-alpha gene, partial cds.  United States: N. p., 2022. 
        Web.  doi:10.82250/3003519.

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                    Opulente, Dana A., & Hittinger, Chris Todd. Pichia sp. yHMH446 translation elongation factor 1-alpha gene, partial cds.  United States.  doi:https://doi.org/10.82250/3003519

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                    Opulente, Dana A., and Hittinger, Chris Todd. 2022.  
"Pichia sp. yHMH446 translation elongation factor 1-alpha gene, partial cds".  United States.  doi:https://doi.org/10.82250/3003519.  https://www.osti.gov/servlets/purl/3003519. Pub date:Wed Jan 19 04:00:00 UTC 2022

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@article{osti_3003519,

  title        = {Pichia sp. yHMH446 translation elongation factor 1-alpha gene, partial cds},

  author       = {Opulente, Dana A. and Hittinger, Chris Todd},

  abstractNote = {A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genus Pichia. Strain yHMH446 falls within a clade that includes Pichia norvegensis, Pichia pseudocactophila, Candida inconspicua, and Pichia cactophila. Whole genome sequence data were analyzed for the presence of genes known to be important for carbon and nitrogen metabolism, and the phenotypic data from the novel species were compared to all Pichia species with publicly available genomes. Across the genus, including the novel species candidate, we found that the inability to use many carbon and nitrogen sources correlated with the absence of metabolic genes. Based on these results, Pichia galeolata sp. nov. is proposed to accommodate yHMH446(T) (=NRRL Y-64187 = CBS 16864). This study shows how integrated taxogenomic analysis can add mechanistic insight to species descriptions.},

  doi          = {10.82250/3003519},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Jan 19 04:00:00 UTC 2022},

  month        = {Wed Jan 19 04:00:00 UTC 2022}

}

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DOI: https://doi.org/10.82250/3003519

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