Pichia sp. strain yHMH446 internal transcribed spacer 2 and large subunit ribosomal RNA gene, partial sequence

Opulente, Dana A.; Hittinger, Chris Todd

doi:10.82250/3003518

Title: Pichia sp. strain yHMH446 internal transcribed spacer 2 and large subunit ribosomal RNA gene, partial sequence

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Abstract

A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genus Pichia. Strain yHMH446 falls within a clade that includes Pichia norvegensis, Pichia pseudocactophila, Candida inconspicua, and Pichia cactophila. Whole genome sequence data were analyzed for the presence of genes known to be important for carbon and nitrogen metabolism, and the phenotypic data from the novel species were compared to all Pichia species with publicly available genomes. Across the genus, including the novel species candidate, we found that the inability to use many carbon and nitrogen sources correlated with the absence of metabolic genes. Based on these results, Pichia galeolata sp. nov. is proposed to accommodate yHMH446(T) (=NRRL Y-64187 = CBS 16864). This study shows how integrated taxogenomic analysis can add mechanistic insight to species descriptions.

Authors:

Opulente, Dana A.; Hittinger, Chris Todd

Biology Department Villanova University, Villanova, Pennsylvania, USA
GLBRC - University of Wisconsin

Publication Date:: Tue Nov 16 23:00:00 EST 2021

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: genome sequence; genotype-phenotype map; novel species; taxogenomics; yeast

OSTI Identifier:: 3003518

DOI:: https://doi.org/10.82250/3003518

Citation Formats


                    Opulente, Dana A., and Hittinger, Chris Todd. Pichia sp. strain yHMH446 internal transcribed spacer 2 and large subunit ribosomal RNA gene, partial sequence.  United States: N. p., 2021. 
        Web.  doi:10.82250/3003518.

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                    Opulente, Dana A., & Hittinger, Chris Todd. Pichia sp. strain yHMH446 internal transcribed spacer 2 and large subunit ribosomal RNA gene, partial sequence.  United States.  doi:https://doi.org/10.82250/3003518

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                    Opulente, Dana A., and Hittinger, Chris Todd. 2021.  
"Pichia sp. strain yHMH446 internal transcribed spacer 2 and large subunit ribosomal RNA gene, partial sequence".  United States.  doi:https://doi.org/10.82250/3003518.  https://www.osti.gov/servlets/purl/3003518. Pub date:Tue Nov 16 23:00:00 EST 2021

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@article{osti_3003518,

  title        = {Pichia sp. strain yHMH446 internal transcribed spacer 2 and large subunit ribosomal RNA gene, partial sequence},

  author       = {Opulente, Dana A. and Hittinger, Chris Todd},

  abstractNote = {A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genus Pichia. Strain yHMH446 falls within a clade that includes Pichia norvegensis, Pichia pseudocactophila, Candida inconspicua, and Pichia cactophila. Whole genome sequence data were analyzed for the presence of genes known to be important for carbon and nitrogen metabolism, and the phenotypic data from the novel species were compared to all Pichia species with publicly available genomes. Across the genus, including the novel species candidate, we found that the inability to use many carbon and nitrogen sources correlated with the absence of metabolic genes. Based on these results, Pichia galeolata sp. nov. is proposed to accommodate yHMH446(T) (=NRRL Y-64187 = CBS 16864). This study shows how integrated taxogenomic analysis can add mechanistic insight to species descriptions.},

  doi          = {10.82250/3003518},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Nov 16 23:00:00 EST 2021},

  month        = {Tue Nov 16 23:00:00 EST 2021}

}

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DOI: https://doi.org/10.82250/3003518

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