Data from: Switchgrass rhizosphere metabolite chemistry driven by nitrogen availability

Smercina, Darian; Bowsher, Alan W.; Evans, Sarah E.; Friesen, Maren L.; Eder, Elizabeth K.; Hoyt, David W.; Tiemann, Lisa K.

doi:10.5061/dryad.9zw3r229b

Title: Data from: Switchgrass rhizosphere metabolite chemistry driven by nitrogen availability

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Abstract

Plants and soil microorganisms interact closely in the rhizosphere where plants may exchange carbon (C) for functional benefits from the microbial community. For example, the bioenergy crop, switchgrass (Panicum virgatum) is thought to exchange root-exuded C for nitrogen (N) fixed by diazotrophs (free-living N-fixers). However, this interaction is not well characterized and it is not known how or if switchgrass responds to diazotrophs or their activity. To explore this question, we assessed rhizosphere metabolite chemistry of switchgrass grown in a hydroponic system under two N levels and under inoculated or uninoculated conditions. Plants were grown with the inoculum Azotobacter vinelandii DJ for three days before harvest. We found switchgrass root exudate chemistry to be driven by N availability. Total metabolite concentrations were generally greater under high N versus low N and unaffected by inoculation. Examination of rhizosphere chemical fingerprints indicates metabolite chemistry was also driven strongly by N availability with a greater relative abundance of carbohydrates under high N and greater relative abundance of organic acids under low N. We also found evidence of changes in rhizosphere chemical fingerprints by inoculation treatment. However, we found little evidence of N treatment and inoculation interaction effects which suggests this response is notmore » directly mediated by N availability.« less

Authors:

Smercina, Darian; Bowsher, Alan W.; Evans, Sarah E.; Friesen, Maren L.; Eder, Elizabeth K.; Hoyt, David W.; Tiemann, Lisa K.

Michigan State Univ., East Lansing, MI (United States); OSTI
Michigan State Univ., East Lansing, MI (United States)
Washington State Univ., Pullman, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Publication Date:: Thu Mar 26 04:00:00 UTC 2020

DOE Contract Number:: SC0014108; SC0018409; FC02-07ER64494; AC02-05CH11231; AC05-76RL01830

Research Org.:: Michigan State Univ., East Lansing, MI (United States); Great Lakes Bioenergy Research Center, Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

OSTI Identifier:: 1874221

DOI:: https://doi.org/10.5061/dryad.9zw3r229b

Citation Formats


                    Smercina, Darian, Bowsher, Alan W., Evans, Sarah E., Friesen, Maren L., Eder, Elizabeth K., Hoyt, David W., and Tiemann, Lisa K. Data from: Switchgrass rhizosphere metabolite chemistry driven by nitrogen availability.  United States: N. p., 2020. 
        Web.  doi:10.5061/dryad.9zw3r229b.

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                    Smercina, Darian, Bowsher, Alan W., Evans, Sarah E., Friesen, Maren L., Eder, Elizabeth K., Hoyt, David W., & Tiemann, Lisa K. Data from: Switchgrass rhizosphere metabolite chemistry driven by nitrogen availability.  United States.  doi:https://doi.org/10.5061/dryad.9zw3r229b

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                    Smercina, Darian, Bowsher, Alan W., Evans, Sarah E., Friesen, Maren L., Eder, Elizabeth K., Hoyt, David W., and Tiemann, Lisa K. 2020.  
"Data from: Switchgrass rhizosphere metabolite chemistry driven by nitrogen availability".  United States.  doi:https://doi.org/10.5061/dryad.9zw3r229b.  https://www.osti.gov/servlets/purl/1874221. Pub date:Thu Mar 26 04:00:00 UTC 2020

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@article{osti_1874221,

  title        = {Data from: Switchgrass rhizosphere metabolite chemistry driven by nitrogen availability},

  author       = {Smercina, Darian and Bowsher, Alan W. and Evans, Sarah E. and Friesen, Maren L. and Eder, Elizabeth K. and Hoyt, David W. and Tiemann, Lisa K.},

  abstractNote = {Plants and soil microorganisms interact closely in the rhizosphere where plants may exchange carbon (C) for functional benefits from the microbial community. For example, the bioenergy crop, switchgrass (Panicum virgatum) is thought to exchange root-exuded C for nitrogen (N) fixed by diazotrophs (free-living N-fixers). However, this interaction is not well characterized and it is not known how or if switchgrass responds to diazotrophs or their activity. To explore this question, we assessed rhizosphere metabolite chemistry of switchgrass grown in a hydroponic system under two N levels and under inoculated or uninoculated conditions. Plants were grown with the inoculum Azotobacter vinelandii DJ for three days before harvest. We found switchgrass root exudate chemistry to be driven by N availability. Total metabolite concentrations were generally greater under high N versus low N and unaffected by inoculation. Examination of rhizosphere chemical fingerprints indicates metabolite chemistry was also driven strongly by N availability with a greater relative abundance of carbohydrates under high N and greater relative abundance of organic acids under low N. We also found evidence of changes in rhizosphere chemical fingerprints by inoculation treatment. However, we found little evidence of N treatment and inoculation interaction effects which suggests this response is not directly mediated by N availability.},

  doi          = {10.5061/dryad.9zw3r229b},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Mar 26 04:00:00 UTC 2020},

  month        = {Thu Mar 26 04:00:00 UTC 2020}

}

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DOI: https://doi.org/10.5061/dryad.9zw3r229b

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