U.S. Department of EnergyOffice of Scientific and Technical Information
Data from: Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient
Abstract
Associative N fixation (ANF), the process by which dinitrogen gas is converted to ammonia by bacteria in casual association with plants, has not been well-studied in temperate ecosystems. We examined the ANF potential of switchgrass (Panicum virgatum L.), a North American prairie grass whose productivity is often unresponsive to N fertilizer addition, via separate short-term 15N2 incubations of rhizosphere soils and excised roots four times during the growing season. Measurements occurred along N fertilization gradients at two sites with contrasting soil fertility (Wisconsin, USA Mollisols and Michigan, USA Alfisols). In general, we found that ANF potentials declined with long-term N addition, corresponding with increased soil N availability. Although we hypothesized that ANF potential would track plant N demand through the growing season, the highest root fixation rates occurred after plants senesced, suggesting that root diazotrophs exploit carbon (C) released during senescence, as C is translocated from aboveground tissues to roots for wintertime storage. Measured ANF potentials, coupled with mass balance calculations, suggest that ANF appears to be an important source of N to unfertilized switchgrass, and, by extension, to temperate grasslands in general.
- Authors:
-
- Michigan State Univ., East Lansing, MI (United States); OSTI
- Univ. of Wisconsin, Madison, WI (United States)
- Michigan State Univ., East Lansing, MI (United States)
- Publication Date:
- DOE Contract Number:
- FC02-07ER64494; AC05-76RL01830
- Research Org.:
- Great Lakes Bioenergy Research Center, Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES
- OSTI Identifier:
- 1873861
- DOI:
- https://doi.org/10.5061/dryad.60bn81v
Citation Formats
Roley, Sarah S., Duncan, David S., Liang, Di, Garoutte, Aaron, Jackson, Randall D., Tiedje, James M., and Robertson, G. Philip. Data from: Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient. United States: N. p., 2019.
Web. doi:10.5061/dryad.60bn81v.
Roley, Sarah S., Duncan, David S., Liang, Di, Garoutte, Aaron, Jackson, Randall D., Tiedje, James M., & Robertson, G. Philip. Data from: Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient. United States. doi:https://doi.org/10.5061/dryad.60bn81v
Roley, Sarah S., Duncan, David S., Liang, Di, Garoutte, Aaron, Jackson, Randall D., Tiedje, James M., and Robertson, G. Philip. 2019.
"Data from: Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient". United States. doi:https://doi.org/10.5061/dryad.60bn81v. https://www.osti.gov/servlets/purl/1873861. Pub date:Wed Dec 04 23:00:00 EST 2019
@article{osti_1873861,
title = {Data from: Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient},
author = {Roley, Sarah S. and Duncan, David S. and Liang, Di and Garoutte, Aaron and Jackson, Randall D. and Tiedje, James M. and Robertson, G. Philip},
abstractNote = {Associative N fixation (ANF), the process by which dinitrogen gas is converted to ammonia by bacteria in casual association with plants, has not been well-studied in temperate ecosystems. We examined the ANF potential of switchgrass (Panicum virgatum L.), a North American prairie grass whose productivity is often unresponsive to N fertilizer addition, via separate short-term 15N2 incubations of rhizosphere soils and excised roots four times during the growing season. Measurements occurred along N fertilization gradients at two sites with contrasting soil fertility (Wisconsin, USA Mollisols and Michigan, USA Alfisols). In general, we found that ANF potentials declined with long-term N addition, corresponding with increased soil N availability. Although we hypothesized that ANF potential would track plant N demand through the growing season, the highest root fixation rates occurred after plants senesced, suggesting that root diazotrophs exploit carbon (C) released during senescence, as C is translocated from aboveground tissues to roots for wintertime storage. Measured ANF potentials, coupled with mass balance calculations, suggest that ANF appears to be an important source of N to unfertilized switchgrass, and, by extension, to temperate grasslands in general.},
doi = {10.5061/dryad.60bn81v},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Dec 04 23:00:00 EST 2019},
month = {Wed Dec 04 23:00:00 EST 2019}
}