XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction

doi:10.11577/1605552

Title: XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction

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Abstract

Room temperature structure of the Influenza A M2 channel wild type transmembrane domain at intermediate pH (pH 6.5)

Publication Date:: Fri Mar 20 00:00:00 EDT 2020

Other Number(s):: CXIDB ID 138

DOE Contract Number:: AC02-05CH11231

Research Org.:: Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Kyoto University

Sponsoring Org.:: Kyoto University

Subject:: BL3; Influenza A M2 channel wild type TM domain at intermediate pH; SACLA; Serial Femtosecond Crystallography; X-ray Free-electorn Lasers; XFEL

OSTI Identifier:: 1605552

DOI:: https://doi.org/10.11577/1605552

Citation Formats


                    XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction.  United States: N. p., 2020. 
        Web.  doi:10.11577/1605552.

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                    XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction.  United States.  doi:https://doi.org/10.11577/1605552

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                    2020.  
"XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction".  United States.  doi:https://doi.org/10.11577/1605552.  https://www.osti.gov/servlets/purl/1605552. Pub date:Fri Mar 20 00:00:00 EDT 2020

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@article{osti_1605552,

  title        = {XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction},

  
  abstractNote = {Room temperature structure of the Influenza A M2 channel wild type transmembrane domain at intermediate pH (pH 6.5)},

  doi          = {10.11577/1605552},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Mar 20 00:00:00 EDT 2020},

  month        = {Fri Mar 20 00:00:00 EDT 2020}

}

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DOI: https://doi.org/10.11577/1605552

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XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction

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XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction

Journal Article Thomaston, Jessica L. ; Woldeyes, Rahel A. ; Nakane, Takanori ; ... - Proceedings of the National Academy of Sciences of the United States of America

The M2 proton channel of influenza A is a drug target that is essential for the reproduction of the flu virus. It is also a model system for the study of selective, unidirectional proton transport across a membrane. Ordered water molecules arranged in “wires” inside the channel pore have been proposed to play a role in both the conduction of protons to the four gating His37 residues and the stabilization of multiple positive charges within the channel. To visualize the solvent in the pore of the channel at room temperature while minimizing the effects of radiation damage, data were collectedmore »« less
XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction

Journal Article Thomaston, Jessica L. ; Woldeyes, Rahel A. ; Nakane, Takanori ; ... - Proceedings of the National Academy of Sciences of the United States of America

Significance The influenza M2 channel is among the smallest proton-selective channels found in nature. High-resolution structures of this channel further our understanding of how protons are transported across a membrane bilayer through a constricted membrane protein environment. This study also has broader implications for the structural interpretation of water in channels using X-ray crystallographic techniques. Previous crystallographic structure determined using synchrotron radiation with cryocooling were biased by the low temperature, and room temperature data collection was limited by radiation damage. These problems have been avoided through room temperature diffraction at an X-ray free-electron laser (XFEL) source.
XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction

Journal Article Thomaston, Jessica L. ; Woldeyes, Rahel A. ; Nakane, Takanori ; ... - Proceedings of the National Academy of Sciences of the United States of America

TheM2 proton channel of influenza A is a drug target that is essential for the reproduction of the flu virus. It is also a model system for the study of selective, unidirectional proton transport across amembrane. Ordered water molecules arranged in "wires" inside the channel pore have been proposed to play a role in both the conduction of protons to the four gating His37 residues and the stabilization of multiple positive charges within the channel. To visualize the solvent in the pore of the channel at room temperature while minimizing the effects of radiation damage, data were collected to amore »« less
Ionization by XFEL radiation produces distinct structure in liquid water

Dataset Stransky, Michal

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