Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Wolff, Alexander

doi:10.11577/1598261

Title: Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Dataset
Other Related Research

Abstract

RT XFEL structure of CypA solved using LCP injection system

Authors:: Wolff, Alexander

Publication Date:: Fri Feb 07 00:00:00 EST 2020

Other Number(s):: CXIDB ID 132

DOE Contract Number:: AC02-05CH11231

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Coherent X-ray Imaging Data Bank; UCSF

Sponsoring Org.:: UCSF

Keywords:: XFEL; X-ray Free-electorn Lasers; Serial Femtosecond Crystallography; Cyclophilin A; MFX; LCLS

OSTI Identifier:: 1598261

DOI:: https://doi.org/10.11577/1598261

Citation Formats


                    Wolff, Alexander. Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals.  United States: N. p., 2020. 
        Web.  doi:10.11577/1598261.

Copy to clipboard


                    Wolff, Alexander. Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals.  United States.  doi:https://doi.org/10.11577/1598261

Copy to clipboard


                    Wolff, Alexander. 2020.  
"Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals".  United States.  doi:https://doi.org/10.11577/1598261.  https://www.osti.gov/servlets/purl/1598261. Pub date:Fri Feb 07 00:00:00 EST 2020

Copy to clipboard


                    
@article{osti_1598261,

  title        = {Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals},

  author       = {Wolff, Alexander},

  abstractNote = {RT XFEL structure of CypA solved using LCP injection system},

  doi          = {10.11577/1598261},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Feb 07 00:00:00 EST 2020},

  month        = {Fri Feb 07 00:00:00 EST 2020}

}

Copy to clipboard

Dataset:

View Dataset

DOI: https://doi.org/10.11577/1598261

Save / Share:

Export Metadata

Save to My Library

Similar records in DOE Data Explorer and OSTI.GOV collections:

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Dataset Wolff, Alexander

RT XFEL structure of CypA solved using MESH injection system
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Dataset Wolff, Alexander

RT XFEL structure of CypA solved using celloluse carrier media
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Journal Article Wolff, Alexander M. ; Young, Iris D. ; Sierra, Raymond G. ; ... - IUCrJ

Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural information from crystals on the nanometre to micrometre scale. Despite the utility of these methods, their implementation can often be difficult, as they present many challenges that are not encountered in traditional macromolecular crystallography experiments. Here, XFEL serial crystallography experiments and MicroED experiments using batch-grown microcrystals of the enzyme cyclophilin A are described. The results provide a roadmap for researchers hoping to design macromolecular microcrystallography experiments, andmore »« less
Cited by 15
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Journal Article Wolff, Alexander M. ; Young, Iris D. ; Sierra, Raymond G. ; ... - IUCrJ

Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural information from crystals on the nanometre to micrometre scale. Despite the utility of these methods, their implementation can often be difficult, as they present many challenges that are not encountered in traditional macromolecular crystallography experiments. Here, XFEL serial crystallography experiments and MicroED experiments using batch-grown microcrystals of the enzyme cyclophilin A are described. The results provide a roadmap for researchers hoping to design macromolecular microcrystallography experiments, andmore »« less
Cited by 15
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Journal Article Wolff, Alexander M. ; Young, Iris D. ; Sierra, Raymond G. ; ... - IUCrJ

Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural information from crystals on the nanometre to micrometre scale. Despite the utility of these methods, their implementation can often be difficult, as they present many challenges that are not encountered in traditional macromolecular crystallography experiments. Here, XFEL serial crystallography experiments and MicroED experiments using batch-grown microcrystals of the enzyme cyclophilin A are described. The results provide a roadmap for researchers hoping to design macromolecular microcrystallography experiments, andmore »« less
Cited by 15

Similar Records