Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

Wolff, Alexander

doi:10.11577/1595660

Title: Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals

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Abstract

RT XFEL structure of CypA solved using celloluse carrier media

Authors:: Wolff, Alexander

Publication Date:: Wed Jan 29 00:00:00 EST 2020

Other Number(s):: CXIDB ID 133

DOE Contract Number:: AC02-05CH11231

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Coherent X-ray Imaging Data Bank; UCSF

Sponsoring Org.:: UCSF

Keywords:: SACLA; XFEL; X-ray Free-electorn Lasers; Serial Femtosecond Crystallography; Cyclophilin A; BL2

OSTI Identifier:: 1595660

DOI:: https://doi.org/10.11577/1595660

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                    Wolff, Alexander. Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals.  United States: N. p., 2020. 
        Web.  doi:10.11577/1595660.

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                    Wolff, Alexander. Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals.  United States.  doi:https://doi.org/10.11577/1595660

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                    Wolff, Alexander. 2020.  
"Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals".  United States.  doi:https://doi.org/10.11577/1595660.  https://www.osti.gov/servlets/purl/1595660. Pub date:Wed Jan 29 00:00:00 EST 2020

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@article{osti_1595660,

  title        = {Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals},

  author       = {Wolff, Alexander},

  abstractNote = {RT XFEL structure of CypA solved using celloluse carrier media},

  doi          = {10.11577/1595660},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Jan 29 00:00:00 EST 2020},

  month        = {Wed Jan 29 00:00:00 EST 2020}

}

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DOI: https://doi.org/10.11577/1595660

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Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural information from crystals on the nanometre to micrometre scale. Despite the utility of these methods, their implementation can often be difficult, as they present many challenges that are not encountered in traditional macromolecular crystallography experiments. Here, XFEL serial crystallography experiments and MicroED experiments using batch-grown microcrystals of the enzyme cyclophilin A are described. The results provide a roadmap for researchers hoping to design macromolecular microcrystallography experiments, andmore »« less
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