Enhanced Spore Biomarker Detection Following Laser Induced Lysis
Matrix assisted laser desorption/ionization (MALDI) has grown in popularity as a means to rapidly analyze proteins directly from bacterial cells. This method provides identifying information by generating protein ?fingerprints? for each organism. However, generating rich protein fingerprints from spores, such as from the genus Bacillus, has proven difficult. We have examined the use of laser energy to induce spore lysis and increase the protein signature complexity. As a measure of lysis, the ions from calcium and dipicolinic acid (DPA) were monitored along with the higher m/z protein ions. DPA is a known marker of eubacterial spores usually as a complex with calcium. This is in contrast to the abundant geogenic calcium complexes with carbonate among other forms. A combination of general bacterial markers, DPA and calcium, and protein fingerprints can be used to provide complementary biomarkers from a single sample preparation.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 992031
- Report Number(s):
- PNNL-SA-36103; 400403809
- Country of Publication:
- United States
- Language:
- English
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