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Biomass and salt-dependent effects of Bacillus spores on radionuclide migration from the Waste Isolation Pilot Plant

Journal Article · · Chemosphere
 [1];  [1];  [2];  [1];  [3]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Texas Tech Univ., Lubbock, TX (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
+ Show Author Affiliations

Spores of a Bacillus sp., isolated from radioactive waste, were tested for their ability to influence the fate and transport of neodymium (Nd3+) under high salt conditions expected at the Waste Isolation Pilot Plant (WIPP) nuclear waste repository. Spores were suspended in neodymium-spiked saline solutions up to 4 M NaCl, and concentrations of Nd and the complexing agent dipicolinic acid (DPA), a component of spores, were monitored along with optical densities and spore numbers. Overall, results support neodymium bioassociation that is dependent upon biomass, with more apparent adsorption occurring at higher spore concentrations. However, probable spore lysis in 2 and 4 M NaCl solutions and possible germination at 0.15 M NaCl appear to drive the release of DPA and subsequent return of Nd to solution. The implications of this work for the WIPP will depend on actual biomass levels and the ionic strength of the repository brines.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
89233218CNA000001
OSTI ID:
1783544
Alternate ID(s):
OSTI ID: 1797511
Report Number(s):
LA-UR--21-22235
Journal Information:
Chemosphere, Journal Name: Chemosphere Vol. 280; ISSN 0045-6535
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES
Bacillus spores
Biological science
WIPP
biological influence
dipicolinic acid
neodymium