Responses of bacterial and archaeal ammonia oxidizers to soil organic and fertilizer amendments under long-term management

Journal Article · Sat May 01 04:00:00 EDT 2010 · Applied Soil Ecology

DOI:https://doi.org/10.1016/j.apsoil.2010.04.003· OSTI ID:989850

Wessen, E; Nyberg, K; Jansson, J K; Hallin, S

Ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) co-exist in soil, but their relative distribution may vary depending on the environmental conditions. Effects of changes in soil organic matter and nutrient content on the AOB and AOA are poorly understood. Our aim was to compare effects of long-term soil organic matter depletion and amendments with labile (straw) and more recalcitrant (peat) organic matter, with and without easily plant-available nitrogen, on the activities, abundances and community structures of AOB and AOA. Soil was sampled from a long-term field site in Sweden that was established in 1956. The potential ammonia oxidation rates, the AOB and AOA amoA gene abundances and the community structures of both groups based on T-RFLP of amoA genes were determined. Straw amendment during 50 years had not altered any of the measured soil parameters, while the addition of peat resulted in a significant increase of soil organic carbon as well as a decrease in pH. Nitrogen fertilization alone resulted in a small decrease in soil pH, organic carbon and total nitrogen, but an increase in primary production. Type and amount of organic matter had an impact on the AOB and AOA community structures and the AOA abundance. Our findings confirmed that AOA are abundant in soil, but showed that under certain conditions the AOB dominate, suggesting niche differentiation between the two groups at the field site. The large differences in potential rates between treatments correlated to the AOA community size, indicating that they were functionally more important in the nitrification process than the AOB. The AOA abundance was positively related to addition of labile organic carbon, which supports the idea that AOA could have alternative growth strategies using organic carbon. The AOB community size varied little in contrast to that of the AOA. This indicates that the bacterial ammonia oxidizers as a group have a greater ecophysiological diversity and potentially cover a broader range of habitats.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: Earth Sciences Division
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 989850
• Report Number(s): LBNL-3651E
• Journal Information: Applied Soil Ecology, Journal Name: Applied Soil Ecology Journal Issue: 3 Vol. 45; ISSN 0929-1393
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
58 GEOSCIENCES
ABUNDANCE
AMMONIA
BACTERIA
CARBON
DISTRIBUTION
FERTILIZATION
FERTILIZERS
GENES
MANAGEMENT
NITRIFICATION
NITROGEN
NUTRIENTS
ORGANIC MATTER
OXIDATION
OXIDIZERS
PEAT
PRODUCTION
SOILS
STRAW