Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode

Journal Article · Wed Sep 10 04:00:00 EDT 2008 · Electroanalysis, 20(18):2040-2046

DOI:https://doi.org/10.1002/elan.200804287· OSTI ID:985032

Lee, Ai Cheng; Liu, Guodong; Heng, Chew-Kiat; Tan, Swee-Nign; Lim, Tit-Meng; Lin, Yuehe

A rapid, simple and sensitive electrochemical assay of horseradish peroxidase (HRP) performed on disposable screen-printed carbon electrode was developed. HRP activities were monitored by square-wave voltammetric (SWV) measuring the electroactive enzymatic product in the presence of o-aminophenol and hydrogen peroxide substrate solution. SWV analysis demonstrated a greater sensitivity and shorter analysis time than the widely used amperometric and differential-pulsed voltammetric methods. The voltammetric characteristics of substrate and enzymatic product as well as the parameters of SWV analysis were optimized. Under optimized conditions, a linear response for HRP from 0.003 - 0.1 U/mL and a detection limit of 0.002 U/mL (1.25×10-15 mol in 25 µL) were obtained with a good precision (RSD = 8%; n = 6). This rapid and sensitive HRP assay with microliters-assay volume could be readily integrated to portable devices and point-of-care (POC) diagnosis applications.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 985032
• Report Number(s): PNNL-SA-61143; 24822; 400412000
• Journal Information: Electroanalysis, 20(18):2040-2046, Journal Name: Electroanalysis, 20(18):2040-2046 Journal Issue: 18 Vol. 20; ISSN 1521-4109; ISSN 1040-0397
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
ACCURACY
CARBON
DETECTION
DIAGNOSIS
ELECTRODES
Environmental Molecular Sciences Laboratory
HYDROGEN PEROXIDE
PEROXIDASES
SENSITIVITY
SUBSTRATES
electrochemical sensor
horseradish peroxidase
point-of-care.