Bioelectrochemical Magnetic Immunosensing of Trichloropyridinol: A Potential Insecticide Biomarker
A magnetic beads-based bioelectrochemical magnetic immunosensor was developed for the fast and sensitive determination of the trichloropyridinol (TCP) biomarker in environmental samples. After liquid phrase competitive immunoreaction among a limited amount of TCP antibody coated-magnetic beads (Ab-MBs), TCP analyte, and horseradish peroxidase (HRP) labeled TCP (HRP-TCP), a magnet/glassy carbon (MGC) electrode was used to collect a TCP-Abs-MBs and a HRP-TCP-Ab-MBs immunocomplex assembly. The activity of HRP tracers bound to the beads was monitored with highly sensitive square wave voltammetry (SWV) by accumulating an electroactive enzymatic product to the MGC electrode surface under constant potential (0.5 V) during enzymatic reaction in the presence of 3’,3’,5’,5’-tetramethylbenzidine (TMB)-H2O2 substrate solution. The electrochemical characteristics of substrate and product were investigated, and the parameters of the immunoassay were optimized.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 889040
- Report Number(s):
- PNNL-SA-45459; 12490
- Journal Information:
- Electroanalysis, 18(16):1605-1613, Journal Name: Electroanalysis, 18(16):1605-1613 Journal Issue: 16 Vol. 18
- Country of Publication:
- United States
- Language:
- English
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