Using a Multijunction Microfluidic Device to Inject Substrate inot an Array of Preformed Plugs Without Cross-Contamination: Comparing Theory and Experiments
Journal Article
·
· Analytical Chemistry (Washington)
OSTI ID:980671
In this paper we describe a multijunction microfluidic device for the injection of a substrate into an array of preformed plugs carried by an immiscible fluid in a microchannel. The device uses multiple junctions to inject substrate into preformed plugs without synchronization of the flow of substrate and the array of preformed plugs of reagent, which reduces cross-contamination of the plugs, eliminates formation of small droplets of substrate, and allows a greater range of injection ratios compared to that of a single T-junction. The device was based on a previously developed physical model for transport that was here adapted to describe injection and experimentally verified. After characterization, the device was applied to two biochemical assays, including evaluation of the enzymatic activity of thrombin and determination of the coagulation time of human blood plasma, which both provided reliable results. The reduction of cross-contamination and greater range of injection ratios achieved by this device may improve the processes that involve addition and titration of reagents into plugs, such as high-throughput screening of protein crystallization conditions.
- Research Organization:
- Brookhaven National Laboratory (BNL) National Synchrotron Light Source
- Sponsoring Organization:
- Doe - Office Of Science
- DOE Contract Number:
- AC02-98CH10886
- OSTI ID:
- 980671
- Report Number(s):
- BNL--93589-2010-JA
- Journal Information:
- Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 7 Vol. 79; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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