Combining Electron Crystallography and X-ray Crystallography to Study the MlotiK1 Cyclic Nucleotide-Regulated Potassium Channel

Journal Article · Thu Jan 01 04:00:00 EST 2009 · Journal of Structural Biology

DOI:https://doi.org/10.1016/j.jsb.2009.06.012· OSTI ID:980073

Clayton, G; Aller, S; Wang, J; Unger, V; Morais-Cabral, J

We have recently reported the X-ray structure of the cyclic nucleotide-regulated potassium channel, MlotiK1. Here we describe the application of both electron and X-ray crystallography to obtain high quality crystals. We suggest that the combined application of these techniques provides a useful strategy for membrane protein structure determination. We also present negative stain projection and cryo-data projection maps. These maps provide new insights about the properties of the MlotiK1 channel. In particular, a comparison of a 9 {angstrom} cryo-data projection with calculated model maps strongly suggests that there is a very weak interaction between the pore and the S1-S4 domains of this 6 TM tetrameric cation channel and that the S1-S4 domains can adopt multiple orientations relative to the pore.

• Research Organization: Brookhaven National Laboratory (BNL) National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 980073
• Report Number(s): BNL--92991-2010-JA
• Journal Information: Journal of Structural Biology, Journal Name: Journal of Structural Biology Journal Issue: 3 Vol. 167; ISSN JSBIEM; ISSN 1047-8477
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
CATIONS
CRYSTALLOGRAPHY
CRYSTALS
ELECTRONS
MAPS
MEMBRANE PROTEINS
POTASSIUM
STAINS
USES
WEAK INTERACTIONS
national synchrotron light source