Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels

Shuart, Noah G.; Haitin, Yoni; Camp, Stacey S.; Black, Kevin D.; Zagotta, William N.

doi:10.1038/ncomms1466

Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels

Journal Article · Tue Aug 30 00:00:00 EDT 2011 · Nature Communications

DOI:https://doi.org/10.1038/ncomms1466· OSTI ID:1623877

Shuart, Noah G. ^[1]; Haitin, Yoni ^[2]; Camp, Stacey S. ^[2]; Black, Kevin D. ^[2]; Zagotta, William N. ^[2]

Univ. of Washington, Seattle, WA (United States). School of Medicine. Howard Hughes Medical Inst. Dept. of Physiology and Biophysics; DOE/OSTI
Univ. of Washington, Seattle, WA (United States). School of Medicine. Howard Hughes Medical Inst. Dept. of Physiology and Biophysics

Molecular determinants of ion channel tetramerization are well characterized, but those involved in heteromeric channel assembly are less clearly understood. The heteromeric composition of native channels is often precisely controlled. Cyclic nucleotide-gated (CNG) channels from rod photoreceptors exhibit a 3:1 stoichiometry of CNGA1 and CNGB1 subunits that tunes the channels for their specialized role in phototransduction. Here we show, using electrophysiology, fluorescence, biochemistry, and X-ray crystallography, that the mechanism for this controlled assembly is the formation of a parallel 3-helix coiled-coil domain of the carboxy-terminal leucine zipper region of CNGA1 subunits, constraining the channel to contain three CNGA1 subunits, followed by preferential incorporation of a single CNGB1 subunit. Deletion of the carboxy-terminal leucine zipper domain relaxed the constraint and permitted multiple CNGB1 subunits in the channel. The X-ray crystal structures of the parallel 3-helix coiled-coil domains of CNGA1 and CNGA3 subunits were similar, suggesting that a similar mechanism controls the stoichiometry of cone CNG channels.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1623877

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 2; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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