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Title: Changes in the composition of the human fecal microbiome following bacteriotherapy for recurrent Clostridium difficile-associated diarrhea

Abstract

CDAD is the major known cause of antibiotic-induced diarrhea and colitis, and the disease is thought to result from persistent disruption of commensal gut microbiota. Bacteriotherapy by way of fecal transplantation can be used to treat recurrent CDAD and is thought to re-establish the normal colonic microflora. However, limitations of conventional microbiologic techniques have until recently precluded testing of this idea. In this study we used T-RFLP and 16S rRNA gene sequencing approaches to characterize the bacterial composition of the colonic microflora in a patient suffering from recurrent CDAD, before and after treatment by fecal transplantation from a healthy donor. While the patient's residual colonic microbiota, prior to therapy, was deficient in members of the bacterial divisions-Firmicutes and Bacteriodetes, transplantation had a dramatic impact on the composition of the patient's gut microbiota. By 14 days post transplantation, the fecal bacterial composition of the recipient was highly similar to the donor and was dominated by Bacteroides spp. strains and an uncharacterized butyrate producing bacterium. The change in bacterial composition was accompanied by resolution of the patient's symptoms. The striking similarity of the recipient's and donor's intestinal microbiota following bacteriotherapy suggests that the donor's bacteria quickly occupied their requisite niches, resulting inmore » restoration of both the structure and function of the microbial communities present.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Earth Sciences Division
OSTI Identifier:
979919
Report Number(s):
LBNL-2887E
TRN: US201011%%471
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Journal of Clinical Gastroenterology
Additional Journal Information:
Related Information: Journal Publication Date: 2009
Country of Publication:
United States
Language:
English
Subject:
54; 58; BACTERIA; CLOSTRIDIUM; COMMUNITIES; DIARRHEA; DISEASES; GENES; MICROORGANISMS; PATIENTS; RESOLUTION; STRAINS; SYMPTOMS; TESTING; THERAPY

Citation Formats

Khoruts, A, Dicksved, J, Jansson, J K, and Sadowsky, M J. Changes in the composition of the human fecal microbiome following bacteriotherapy for recurrent Clostridium difficile-associated diarrhea. United States: N. p., 2009. Web. doi:10.1097/MCG.0b013e3181c87e02.
Khoruts, A, Dicksved, J, Jansson, J K, & Sadowsky, M J. Changes in the composition of the human fecal microbiome following bacteriotherapy for recurrent Clostridium difficile-associated diarrhea. United States. https://doi.org/10.1097/MCG.0b013e3181c87e02
Khoruts, A, Dicksved, J, Jansson, J K, and Sadowsky, M J. Sat . "Changes in the composition of the human fecal microbiome following bacteriotherapy for recurrent Clostridium difficile-associated diarrhea". United States. https://doi.org/10.1097/MCG.0b013e3181c87e02. https://www.osti.gov/servlets/purl/979919.
@article{osti_979919,
title = {Changes in the composition of the human fecal microbiome following bacteriotherapy for recurrent Clostridium difficile-associated diarrhea},
author = {Khoruts, A and Dicksved, J and Jansson, J K and Sadowsky, M J},
abstractNote = {CDAD is the major known cause of antibiotic-induced diarrhea and colitis, and the disease is thought to result from persistent disruption of commensal gut microbiota. Bacteriotherapy by way of fecal transplantation can be used to treat recurrent CDAD and is thought to re-establish the normal colonic microflora. However, limitations of conventional microbiologic techniques have until recently precluded testing of this idea. In this study we used T-RFLP and 16S rRNA gene sequencing approaches to characterize the bacterial composition of the colonic microflora in a patient suffering from recurrent CDAD, before and after treatment by fecal transplantation from a healthy donor. While the patient's residual colonic microbiota, prior to therapy, was deficient in members of the bacterial divisions-Firmicutes and Bacteriodetes, transplantation had a dramatic impact on the composition of the patient's gut microbiota. By 14 days post transplantation, the fecal bacterial composition of the recipient was highly similar to the donor and was dominated by Bacteroides spp. strains and an uncharacterized butyrate producing bacterium. The change in bacterial composition was accompanied by resolution of the patient's symptoms. The striking similarity of the recipient's and donor's intestinal microbiota following bacteriotherapy suggests that the donor's bacteria quickly occupied their requisite niches, resulting in restoration of both the structure and function of the microbial communities present.},
doi = {10.1097/MCG.0b013e3181c87e02},
url = {https://www.osti.gov/biblio/979919}, journal = {Journal of Clinical Gastroenterology},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {8}
}