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Title: The Genome of the Obligately Intracellular Bacterium Ehrlichia canis Reveals Themes of Complex Membrane Structure and Immune Evasion Strategies

Abstract

Ehrlichia canis, a small obligately intracellular, tick-transmitted, gram-negative, {alpha}-proteobacterium, is the primary etiologic agent of globally distributed canine monocytic ehrlichiosis. Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome of 1,315,030 bp predicted to encode 925 proteins, 40 stable RNA species, 17 putative pseudogenes, and a substantial proportion of noncoding sequence (27%). Interesting genome features include a large set of proteins with transmembrane helices and/or signal sequences and a unique serine-threonine bias associated with the potential for O glycosylation that was prominent in proteins associated with pathogen-host interactions. Furthermore, two paralogous protein families associated with immune evasion were identified, one of which contains poly(G-C) tracts, suggesting that they may play a role in phase variation and facilitation of persistent infections. Genes associated with pathogen-host interactions were identified, including a small group encoding proteins (n = 12) with tandem repeats and another group encoding proteins with eukaryote-like ankyrin domains (n = 7).

Authors:
 [1];  [2];  [1];  [1];  [1];  [3];  [1];  [4];  [3];  [1];  [1];  [3];  [1];  [2];  [2];  [2];  [1]
  1. U.S. Department of Energy, Joint Genome Institute
  2. Center for Biodenfense and Emerging Infectious Diseases
  3. ORNL
  4. Lawrence Livermore National Laboratory (LLNL)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
978222
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Bacteriology; Journal Volume: 188; Journal Issue: 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; BACTERIA; CHROMOSOMES; DOGS; GENES; MEMBRANES; PROTEINS; RNA

Citation Formats

Mavromatis, K, Doyle, C Kuyler, Lykidis, A, Ivanova, N, Francino, M P, Chain, Patrick S, Shin, M, Malfatti, Stephanie, Larimer, Frank W, Copeland, A, Detter, J C, Land, Miriam L, Richardson, P M, Yu, X J, Walker, D H, McBride, J W, and Kyripides, N C. The Genome of the Obligately Intracellular Bacterium Ehrlichia canis Reveals Themes of Complex Membrane Structure and Immune Evasion Strategies. United States: N. p., 2006. Web. doi:10.1128/JB.01837-05.
Mavromatis, K, Doyle, C Kuyler, Lykidis, A, Ivanova, N, Francino, M P, Chain, Patrick S, Shin, M, Malfatti, Stephanie, Larimer, Frank W, Copeland, A, Detter, J C, Land, Miriam L, Richardson, P M, Yu, X J, Walker, D H, McBride, J W, & Kyripides, N C. The Genome of the Obligately Intracellular Bacterium Ehrlichia canis Reveals Themes of Complex Membrane Structure and Immune Evasion Strategies. United States. doi:10.1128/JB.01837-05.
Mavromatis, K, Doyle, C Kuyler, Lykidis, A, Ivanova, N, Francino, M P, Chain, Patrick S, Shin, M, Malfatti, Stephanie, Larimer, Frank W, Copeland, A, Detter, J C, Land, Miriam L, Richardson, P M, Yu, X J, Walker, D H, McBride, J W, and Kyripides, N C. Sun . "The Genome of the Obligately Intracellular Bacterium Ehrlichia canis Reveals Themes of Complex Membrane Structure and Immune Evasion Strategies". United States. doi:10.1128/JB.01837-05.
@article{osti_978222,
title = {The Genome of the Obligately Intracellular Bacterium Ehrlichia canis Reveals Themes of Complex Membrane Structure and Immune Evasion Strategies},
author = {Mavromatis, K and Doyle, C Kuyler and Lykidis, A and Ivanova, N and Francino, M P and Chain, Patrick S and Shin, M and Malfatti, Stephanie and Larimer, Frank W and Copeland, A and Detter, J C and Land, Miriam L and Richardson, P M and Yu, X J and Walker, D H and McBride, J W and Kyripides, N C},
abstractNote = {Ehrlichia canis, a small obligately intracellular, tick-transmitted, gram-negative, {alpha}-proteobacterium, is the primary etiologic agent of globally distributed canine monocytic ehrlichiosis. Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome of 1,315,030 bp predicted to encode 925 proteins, 40 stable RNA species, 17 putative pseudogenes, and a substantial proportion of noncoding sequence (27%). Interesting genome features include a large set of proteins with transmembrane helices and/or signal sequences and a unique serine-threonine bias associated with the potential for O glycosylation that was prominent in proteins associated with pathogen-host interactions. Furthermore, two paralogous protein families associated with immune evasion were identified, one of which contains poly(G-C) tracts, suggesting that they may play a role in phase variation and facilitation of persistent infections. Genes associated with pathogen-host interactions were identified, including a small group encoding proteins (n = 12) with tandem repeats and another group encoding proteins with eukaryote-like ankyrin domains (n = 7).},
doi = {10.1128/JB.01837-05},
journal = {Journal of Bacteriology},
number = 11,
volume = 188,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}