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Title: The genome of obligately intracellular Ehrlichia canis revealsthemes of complex membrane structure and immune evasion strategies

Abstract

Ehrlichia canis, a small obligately intracellular, tick-transmitted, gram-negative, a-proteobacterium is the primary etiologic agent of globally distributed canine monocytic ehrlichiosis. Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome of 1,315,030 bp predicted to encode 925 proteins, 40 stable RNA species, and 17 putative pseudogenes, and a substantial proportion of non-coding sequence (27 percent). Interesting genome features include a large set of proteins with transmembrane helices and/or signal sequences, and a unique serine-threonine bias associated with the potential for O-glycosylation that was prominent in proteins associated with pathogen-host interactions. Furthermore, two paralogous protein families associated with immune evasion were identified, one of which contains poly G:C tracts, suggesting that they may play a role in phase variation and facilitation of persistent infections. Proteins associated with pathogen-host interactions were identified including a small group of proteins (12) with tandem repeats and another with eukaryotic-like ankyrin domains (7).

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director. Office of Science. Office of Biological andEnvironmental Research, Lawrence Livermore National Laboratory, LosAlamos National Laboratory; Clayton Foundation for Research, Universityof Texas Medical Branch Sealy Center for Vaccine Development
OSTI Identifier:
887440
Report Number(s):
LBNL-58778
Journal ID: ISSN 0021-9193; JOBAAY; R&D Project: 626869; BnR: KP1103010; TRN: US200618%%186
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Bacteriology; Journal Volume: 188; Journal Issue: 11; Related Information: Journal Publication Date: 06/2006
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMOSOMES; DOGS; MEMBRANES; PROTEINS; RNA; genome Ehrlichia canis immune evasion

Citation Formats

Mavromatis, K., Kuyler Doyle, C., Lykidis, A., Ivanova, N., Francino, P., Chain, P., Shin, M., Malfatti, S., Larimer, F., Copeland,A., Detter, J.C., Land, M., Richardson, P.M., Yu, X.J., Walker, D.H., McBride, J.W., and Kyrpides, N.C. The genome of obligately intracellular Ehrlichia canis revealsthemes of complex membrane structure and immune evasion strategies. United States: N. p., 2005. Web.
Mavromatis, K., Kuyler Doyle, C., Lykidis, A., Ivanova, N., Francino, P., Chain, P., Shin, M., Malfatti, S., Larimer, F., Copeland,A., Detter, J.C., Land, M., Richardson, P.M., Yu, X.J., Walker, D.H., McBride, J.W., & Kyrpides, N.C. The genome of obligately intracellular Ehrlichia canis revealsthemes of complex membrane structure and immune evasion strategies. United States.
Mavromatis, K., Kuyler Doyle, C., Lykidis, A., Ivanova, N., Francino, P., Chain, P., Shin, M., Malfatti, S., Larimer, F., Copeland,A., Detter, J.C., Land, M., Richardson, P.M., Yu, X.J., Walker, D.H., McBride, J.W., and Kyrpides, N.C. Thu . "The genome of obligately intracellular Ehrlichia canis revealsthemes of complex membrane structure and immune evasion strategies". United States. https://www.osti.gov/servlets/purl/887440.
@article{osti_887440,
title = {The genome of obligately intracellular Ehrlichia canis revealsthemes of complex membrane structure and immune evasion strategies},
author = {Mavromatis, K. and Kuyler Doyle, C. and Lykidis, A. and Ivanova, N. and Francino, P. and Chain, P. and Shin, M. and Malfatti, S. and Larimer, F. and Copeland,A. and Detter, J.C. and Land, M. and Richardson, P.M. and Yu, X.J. and Walker, D.H. and McBride, J.W. and Kyrpides, N.C.},
abstractNote = {Ehrlichia canis, a small obligately intracellular, tick-transmitted, gram-negative, a-proteobacterium is the primary etiologic agent of globally distributed canine monocytic ehrlichiosis. Complete genome sequencing revealed that the E. canis genome consists of a single circular chromosome of 1,315,030 bp predicted to encode 925 proteins, 40 stable RNA species, and 17 putative pseudogenes, and a substantial proportion of non-coding sequence (27 percent). Interesting genome features include a large set of proteins with transmembrane helices and/or signal sequences, and a unique serine-threonine bias associated with the potential for O-glycosylation that was prominent in proteins associated with pathogen-host interactions. Furthermore, two paralogous protein families associated with immune evasion were identified, one of which contains poly G:C tracts, suggesting that they may play a role in phase variation and facilitation of persistent infections. Proteins associated with pathogen-host interactions were identified including a small group of proteins (12) with tandem repeats and another with eukaryotic-like ankyrin domains (7).},
doi = {},
journal = {Journal of Bacteriology},
number = 11,
volume = 188,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 2005},
month = {Thu Sep 01 00:00:00 EDT 2005}
}