The mapping of novel genes to human chromosome 19

Technical Report · Thu Dec 01 04:00:00 EST 1994

DOI:https://doi.org/10.2172/96636· OSTI ID:96636

Buenaventura, J. M. ^[1]

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1. Sarah Lawrence College, Bronxville, NY (United States)

The principle goal of our laboratory is the discovery of new genes on human chromosome 19. One of the strategies to achieve this goal is through the use of cDNA clones known as {open_quotes}expressed sequence tags{close_quotes} (ESTs). ESTs, short segments of sequence from a cDNA clone that correspond to the mRNA, occur as unique regions in the genome and, therefore, can be used as markers for specific positions. In collaboration with researchers from Genethon in France, fifteen cDNA clones from a normalized human infant brain cDNA library were tested and determined to map to chromosome 19. A verification procedure is then followed to confirm assignment to chromosome 19. First, primers for each cDNA clone are developed and then amplified by polymerase chain reaction from genomic DNA. Next, a {sup 32}P-radiolabeled probe is made by polymerase chain reaction for each clone and then hybridized against filters containing an LLNL chromosome 19-specific cosmid library to find putative locations on the chromosome. The location is then verified by running a polymerase chain reactions from the positive cosmids. With the Browser database at LLNL, additional information about the positive cosmids can be found. Through use of the BLAST database at the National Library of Medicine, homologous sequences to the clones can be found. Among the fifteen cDNA clones received from Genethon, all have been amplified by polymerase chain reaction. Three have turned out as repetitive elements in the genome. Ten have been mapped to specific locations on chromosome 19. Putative locations have been found for the remaining two clones and thus verification testing will proceed.

• Research Organization: Lawrence Livermore National Lab., CA (United States)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 96636
• Report Number(s): UCRL-ID--114972-3; ON: DE95009444
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CLONING
DNA HYBRIDIZATION
GENE AMPLIFICATION
GENE MUTATIONS
GENETIC MAPPING
HUMAN CHROMOSOME 19
LABELLED COMPOUNDS
PHOSPHORUS 32
POLYMERASE CHAIN REACTION